| Autor: |
Kim HS; College of Life Science, Gangneung-Wonju National University, 7 Jukheon-gil, Gangneung, Gangwon 25457, Republic of Korea., Ahn JW; College of Life Science, Gangneung-Wonju National University, 7 Jukheon-gil, Gangneung, Gangwon 25457, Republic of Korea., Ha NR; Huscion MAJIC R&D Center, 331 Pangyo-ro, Seongnam, Gyeonggi 13488, Republic of Korea., Damodar K; Huscion MAJIC R&D Center, 331 Pangyo-ro, Seongnam, Gyeonggi 13488, Republic of Korea., Jang SK; Huscion MAJIC R&D Center, 331 Pangyo-ro, Seongnam, Gyeonggi 13488, Republic of Korea., Yoo YM; College of Life Science, Gangneung-Wonju National University, 7 Jukheon-gil, Gangneung, Gangwon 25457, Republic of Korea., Gyoung YS; Huscion MAJIC R&D Center, 331 Pangyo-ro, Seongnam, Gyeonggi 13488, Republic of Korea., Joo SS; College of Life Science, Gangneung-Wonju National University, 7 Jukheon-gil, Gangneung, Gangwon 25457, Republic of Korea.; Huscion MAJIC R&D Center, 331 Pangyo-ro, Seongnam, Gyeonggi 13488, Republic of Korea. |
| Abstrakt: |
Atopic dermatitis (AD) is a chronic skin condition that is characterized by dysregulated immune responses and a heightened risk of Staphylococcus aureus infections, necessitating the advancement of innovative therapeutic methods. This study explored the potential of (6Z,9Z,12Z,15Z)-(2R,3R,4R,5R)-2,3,4,5,6-pentahydroxyhexyl octadeca-6,9,12,15-tetraenoate (HSN-S1), a compound derived from the marine alga Hizikia fusiformis , which shows anti-inflammatory, antimicrobial, and immunomodulatory properties. HSN-S1 was isolated and characterized using advanced chromatographic and spectroscopic methods. Its efficacy was evaluated via in vitro assays with keratinocytes, macrophages, and T cells to assess cytokine suppression and its immunomodulatory effects; its antibacterial activity against S. aureus was quantified. The in vivo effectiveness was validated using a 2,4-dinitrochlorobenzene-induced AD mouse model that focused on skin pathology and cytokine modulation. HSN-S1 significantly reduced pro-inflammatory cytokine secretion, altered T-helper cell cytokine profiles, and showed strong antibacterial activity against S. aureus . In vivo, HSN-S1 alleviated AD-like symptoms in mice and reduced skin inflammation, transepidermal water loss, serum immunoglobulin-E levels, and Th2/Th17 cytokine outputs. These findings suggest HSN-S1 to be a promising marine-derived candidate for AD treatment, as it offers a dual-target approach that could overcome the limitations of existing therapies, hence warranting further clinical investigation. |
