| Autor: |
Procel N; Cancer Research Group, Faculty of Engineering and Applied Sciences, Universidad de Las Américas, Quito 170124, Ecuador., Camacho K; Cancer Research Group, Faculty of Engineering and Applied Sciences, Universidad de Las Américas, Quito 170124, Ecuador., Verboven E; Department of Cell and Molecular Biology, Karolinska Institutet, 17165 Stockholm, Sweden.; VIB Center for Cancer Biology and KU Leuven Department of Oncology, KU Leuven, 3000 Leuven, Belgium., Baroja I; Cancer Research Group, Faculty of Engineering and Applied Sciences, Universidad de Las Américas, Quito 170124, Ecuador.; Faculty of Health Sciences and Medicine, Universidad de Extremadura, 06800 Mérida, Spain., Guerrero PA; Cancer Research Group, Faculty of Engineering and Applied Sciences, Universidad de Las Américas, Quito 170124, Ecuador., Hillen H; VIB Center for Cancer Biology and KU Leuven Department of Oncology, KU Leuven, 3000 Leuven, Belgium., Estrella-García C; Cancer Research Group, Faculty of Engineering and Applied Sciences, Universidad de Las Américas, Quito 170124, Ecuador., Vizcaíno-Rodríguez N; Cancer Research Group, Faculty of Engineering and Applied Sciences, Universidad de Las Américas, Quito 170124, Ecuador., Sansores-Garcia L; VIB Center for Cancer Biology and KU Leuven Department of Oncology, KU Leuven, 3000 Leuven, Belgium., Santamaría-Naranjo A; Laboratorios Multidisciplinarios de Ciencias Biológicas y Químicas, Universidad de Las Américas, Quito 170513, Ecuador., Romero-Carvajal A; Escuela de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador, Quito 170525, Ecuador., Caicedo A; Colegio de Ciencias de la Salud, Escuela de Medicina, Universidad San Francisco de Quito USFQ, Quito 170901, Ecuador., Halder G; VIB Center for Cancer Biology and KU Leuven Department of Oncology, KU Leuven, 3000 Leuven, Belgium., Moya IM; Cancer Research Group, Faculty of Engineering and Applied Sciences, Universidad de Las Américas, Quito 170124, Ecuador. |
| Abstrakt: |
Tracking cell death in vivo can enable a better understanding of the biological mechanisms underlying tissue homeostasis and disease. Unfortunately, existing cell death labeling methods lack compatibility with in vivo applications or suffer from low sensitivity, poor tissue penetration, and limited temporal resolution. Here, we fluorescently labeled dead cells in vivo with Trypan Blue (TBlue) to detect single scattered dead cells or to generate whole-mount three-dimensional maps of large areas of necrotic tissue during organ regeneration. TBlue effectively marked different types of cell death, including necrosis induced by CCl 4 intoxication in the liver, necrosis caused by ischemia-reperfusion in the skin, and apoptosis triggered by BAX overexpression in hepatocytes. Moreover, due to its short circulating lifespan in blood, TBlue labeling allowed in vivo "pulse and chase" tracking of two temporally spaced populations of dying hepatocytes in regenerating mouse livers. Additionally, upon treatment with cisplatin, TBlue labeled dead cancer cells in livers with cholangiocarcinoma and dead thymocytes due to chemotherapy-induced toxicity, showcasing its utility in assessing anticancer therapies in preclinical models. Thus, TBlue is a sensitive and selective cell death marker for in vivo applications, facilitating the understanding of the fundamental role of cell death in normal biological processes and its implications in disease. |
