| Autor: |
Arriero-Cabañero A; Laboratorio de Regeneración Neural, Hospital Nacional de Parapléjicos, 45071 Toledo, Spain., García-Vences E; Facultad de Ciencias de la Salud, Centro de Investigación en Ciencias de la Salud (CICSA), Universidad Anáhuac México Norte, Huixquilucan 52786, Mexico.; Secretaría de la Defensa Nacional, Escuela Militar de Graduados en Sanidad, Ciudad de Méxcio 11200, Mexico., Sánchez-Torres S; Instituto Mexicano del Seguro Social, Unidad de Investigación Médica en Enfermedades Neurológicas, Hospital de Especialidades, Centro Médico Nacional Siglo XXI. Av. Cuauhtémoc 330, Col. Doctores, Mexico City 06720, Mexico., Aristizabal-Hernandez S; Laboratorio de Regeneración Neural, Hospital Nacional de Parapléjicos, 45071 Toledo, Spain., García-Rama C; Laboratorio de Regeneración Neural, Hospital Nacional de Parapléjicos, 45071 Toledo, Spain., Pérez-Rizo E; Unidad de Ingeniería y Evaluación Motora del Hospital Nacional de Parapléjicos, 45071 Toledo, Spain., Fernández-Mayoralas A; Instituto de Química Orgánica General, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain., Grijalva I; Instituto Mexicano del Seguro Social, Unidad de Investigación Médica en Enfermedades Neurológicas, Hospital de Especialidades, Centro Médico Nacional Siglo XXI. Av. Cuauhtémoc 330, Col. Doctores, Mexico City 06720, Mexico., Buzoianu-Anguiano V; Laboratorio de Regeneración Neural, Hospital Nacional de Parapléjicos, 45071 Toledo, Spain., Doncel-Pérez E; Laboratorio de Regeneración Neural, Hospital Nacional de Parapléjicos, 45071 Toledo, Spain., Mey J; Laboratorio de Regeneración Neural, Hospital Nacional de Parapléjicos, 45071 Toledo, Spain.; EURON Graduate School of Neuroscience, 6229ER Maastricht, The Netherlands. |
| Abstrakt: |
Following spinal cord injury (SCI), the regenerative capacity of the central nervous system (CNS) is severely limited by the failure of axonal regeneration. The regeneration of CNS axons has been shown to occur by grafting predegenerated peripheral nerves (PPNs) and to be promoted by the transplantation of neural precursor cells (NPCs). The introduction of a combinatorial treatment of PPNs and NPCs after SCI has to address the additional problem of glial scar formation, which prevents regenerating axons from leaving the implant and making functional connections. Previously, we discovered that the synthetic sulfoglycolipid Tol-51 inhibits astrogliosis. The objective was to evaluate axonal regeneration and locomotor function improvement after SCI in rats treated with a combination of PPN, NPC, and Tol-51. One month after SCI, the scar tissue was removed and replaced with segments of PPN or PPN+Tol-51; PPN+NPC+Tol-51. The transplantation of a PPN segment favors regenerative axonal growth; in combination with Tol-51 and NPC, 30% of the labeled descending corticospinal axons were able to grow through the PPN and penetrate the caudal spinal cord. The animals treated with PPN showed significantly better motor function. Our data demonstrate that PPN implants plus NPC and Tol-51 allow successful axonal regeneration in the CNS. |
