| Autor: |
Chacón-Fuentes M; Agriaquaculture Nutritional Genomic Center, CGNA, Las Heras 350, Temuco 4780000, Chile., Martínez-Cisterna D; Centro de Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA), Universidad de La Frontera, Av. Francisco Salazar 01145, Casilla 54-D, Temuco 4811230, Chile.; Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, s/n, P.O. Box 58-D, Temuco 4780000, Chile., Lizama M; Programa de Doctorado en Ciencias Agroalimentarias y Medioambiente, Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, Av. Francisco Salazar 01145, Casilla 54-D, Temuco 4811230, Chile.; Escuela de Universidad de Valladolid Doctorado (ESDUVa), Departamento Producción Vegetal y Recursos Forestales, Escuela Técnica Superior de Ingenierías Agrarias, Campus 'La Yutera', Avda. de Madrid, 50, 34004 Palencia, Spain., Asencio-Cancino V; Carrera de Ingenieria en Recursos Naturales, Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, Temuco 4811230, Chile., Matamala I; Programa de Doctorado en Ciencias Agroalimentarias y Medioambiente, Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, Av. Francisco Salazar 01145, Casilla 54-D, Temuco 4811230, Chile.; Departamento de Producción Agropecuaria, Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, Temuco 4811230, Chile., Bardehle L; Centro de Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA), Universidad de La Frontera, Av. Francisco Salazar 01145, Casilla 54-D, Temuco 4811230, Chile.; Departamento de Producción Agropecuaria, Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, Temuco 4811230, Chile. |
| Abstrakt: |
Exploitation of the symbiotic relationship between endophytic fungi and ryegrass is a crucial technique for reducing the incidence of insect pests. This is primarily due to the production of alkaloids, such as peramine, by the fungi. This alkaloid has been reported as both a deterrent and toxic to a variety of insects. However, insects have developed various strategies to counteract plant defenses. One of the most studied methods is their ability to sequester toxic compounds from plants. In this study, we examined the feeding preferences and adaptation to peramine in Chilesia rudis , a native Chilean larva. Using a no-choice assay, we assessed larval feeding preferences and mass gain on seven experimental lines and two commercial cultivars of endophyte-infected and non-infected ryegrass. Pupal development time and adult performance were evaluated post-assay. Additionally, we measured peramine content in larval carcasses, feces, and ryegrass leaves. Jumbo was the most preferred cultivar with 32 mm 2 of leaf tissues consumed. The longest pupal development time was observed in L161 and ALTO AR1, both at 28 days. Wing length in adults was greatest in the Jumbo and L163 cultivars, measuring 1.25 cm and 1.32 cm, respectively. Peramine concentrations were detected in the bodies of C. rudis . In conclusion, this larva can adapt to endophyte-infected ryegrass and develop counter-adaptation mechanisms to mitigate the effects of peramine. |
