| Autor: |
Ramos KA; Faculty of Medicine, Federal University of Jequitinhonha and Mucuri Valleys, Teófilo Otoni 39803-371, MG, Brazil., Soares IGM; Faculty of Medicine, Federal University of Jequitinhonha and Mucuri Valleys, Teófilo Otoni 39803-371, MG, Brazil., Oliveira LMA; Faculty of Medicine, Federal University of Jequitinhonha and Mucuri Valleys, Teófilo Otoni 39803-371, MG, Brazil., Braga MA; Faculty of Medicine, Federal University of Jequitinhonha and Mucuri Valleys, Teófilo Otoni 39803-371, MG, Brazil., Soares PPC; Faculty of Medicine, Federal University of Jequitinhonha and Mucuri Valleys, Teófilo Otoni 39803-371, MG, Brazil., Guarneire GJ; Faculty of Medicine, Federal University of Jequitinhonha and Mucuri Valleys, Teófilo Otoni 39803-371, MG, Brazil., Scherrer EC; Institute of Life Sciences, Federal University of Juiz de Fora, Governador Valadares 35010-177, MG, Brazil., Silva FS; Institute of Life Sciences, Federal University of Juiz de Fora, Governador Valadares 35010-177, MG, Brazil., Lima NM; Department of Chemistry, Federal University of Goiás, Goiânia 74690-900, GO, Brazil., La Porta FA; Department of Chemistry, Federal University of Technology-Paraná, Londrina 86036-370, PR, Brazil., de Jesus A S Andrade T; Nucleus of Applied Research to Sciences (NIAC), Federal Institute of Maranhão, Campus Presidente Dutra, Presidente Dutra 65760-000, MA, Brazil., Preet G; Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Aberdeen AB24 3UE, Scotland, UK., Castro SBR; Institute of Life Sciences, Federal University of Juiz de Fora, Governador Valadares 35010-177, MG, Brazil., Alves CCS; Faculty of Medicine, Federal University of Jequitinhonha and Mucuri Valleys, Teófilo Otoni 39803-371, MG, Brazil., Carli AP; Faculty of Medicine, Federal University of Jequitinhonha and Mucuri Valleys, Teófilo Otoni 39803-371, MG, Brazil. |
| Abstrakt: |
This study aimed to evaluate the efficacy of the ethanolic extract of Anadenanthera colubrina in modulating the immune response in the Experimental Autoimmune Encephalomyelitis (EAE) model. The ethanolic extract of the dried bark was analyzed by ESI (+) Orbitrap-MS to obtain a metabolite profile, demonstrating a wide variety of polyphenols, such as flavonoids and phenolic acids. Various parameters were evaluated, such as clinical signs, cytokines, cellular profile, and histopathology in the central nervous system (CNS). The ethanolic extract of A. colubrina demonstrated significant positive effects attenuating the clinical signs and pathological processes associated with EAE. The beneficial effects of the extract treatment were evidenced by reduced levels of pro-inflammatory cytokines, such as IL1β, IL-6, IL-12, TNF, IFN-γ, and a notable decrease in several cell profiles, including CD8+, CD4+, CD4+IFN-γ, CD4+IL-17+, CD11c+MHC-II+, CD11+CD80+, and CD11+CD86+ in the CNS. In addition, histological analysis revealed fewer inflammatory infiltrates and demyelination sites in the spinal cord of mice treated with the extract compared to the control model group. These results showed, for the first time, that the ethanolic extract of A. colubrina exerts a modulatory effect on inflammatory processes, improving clinical signs in EAE, in the acute phase of the disease, which could be further explored as a possible therapeutic alternative. |
