| Autor: |
Machaliński B; Department of General Pathology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland., Oszutowska-Mazurek D; Department of Histology and Embryology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland., Mazurek P; Department of Signal Processing and Multimedia Engineering, West Pomeranian University of Technology in Szczecin, 70-310 Szczecin, Poland., Parafiniuk M; Department of Forensic Medicine, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland., Szumilas P; Department of Social Medicine and Public Health, Pomeranian Medical University in Szczecin, 71-210 Szczecin, Poland., Zawiślak A; Department of Interdisciplinary Dentistry, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland., Zaremba M; Department of General Pathology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland.; Department of Experimental and Clinical Pharmacology, Centre for Preclinical Research (CBP), Medical University of Warsaw, 02-097 Warsaw, Poland., Stecewicz I; Department of General Pathology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland., Zawodny P; Department of General Pathology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland., Wiszniewska B; Department of Histology and Embryology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland. |
| Abstrakt: |
Skin aging is a complex phenomenon influenced by multiple internal and external factors that can lead to significant changes in skin structure, particularly the degradation of key extracellular matrix (ECM) components such as collagen and elastic fibers in the dermis. In this study, we aimed to meticulously assess the morphological changes within these critical fibrous ECM elements in the dermis of the same volunteer at age 47 and 10 years later (2012 to 2022). Using advanced histological staining techniques, we examined the distribution and characteristics of ECM components, including type I collagen, type III collagen, and elastic fibers. Morphological analysis, facilitated by hematoxylin and eosin staining, allowed for an accurate assessment of fiber bundle thickness and a quantification of collagen and elastic fiber areas. In addition, we used the generalized Pareto distribution for histogram modeling to refine our statistical analyses. This research represents a pioneering effort to examine changes in ECM fiber material, specifically within the male dermis over a decade-long period. Our findings reveal substantial changes in the organization of type I collagen within the ECM, providing insight into the dynamic processes underlying skin aging. |
