Metabolite profile in hereditary spastic paraplegia analyzed using magnetic resonance spectroscopy: a cross-sectional analysis in a longitudinal study.

Autor: Montanaro D; U.O. Dipartimentale e Servizio Autonomo di Risonanza Magnetica, Dipartimento di Neuroscienze dell'Età Evolutiva, IRCCS Fondazione Stella Maris, Pisa, Italy., Vavla M; Child and Adolescent Neuropsychiatric Unit, Department of Women's and Children's Health, University Hospital of Padua, Padova, Italy.; Department of Neurorehabilitation, IRCCS E. Medea Scientific Institute, Conegliano, Italy., Frijia F; Bioengineering Unit, Fondazione Toscana G. Monasterio, Pisa, Italy., Coi A; Unit of Epidemiology of Rare Diseases and Congenital Anomalies, Institute of Clinical Physiology, National Research Council, Pisa, Italy., Baratto A; Department of Radiology, S. Maria dei Battuti Hospital- Conegliano, Treviso, Italy., Pasquariello R; U.O. Dipartimentale e Servizio Autonomo di Risonanza Magnetica, Dipartimento di Neuroscienze dell'Età Evolutiva, IRCCS Fondazione Stella Maris, Pisa, Italy., Stefan C; Department of Neurorehabilitation, IRCCS E. Medea Scientific Institute, Conegliano, Italy., Martinuzzi A; Department of Neurorehabilitation, IRCCS E. Medea Scientific Institute, Conegliano, Italy.
Jazyk: angličtina
Zdroj: Frontiers in neuroscience [Front Neurosci] 2024 Aug 13; Vol. 18, pp. 1416093. Date of Electronic Publication: 2024 Aug 13 (Print Publication: 2024).
DOI: 10.3389/fnins.2024.1416093
Abstrakt: Background: Hereditary Spastic Paraplegias (HSP) are genetic neurodegenerative disorders affecting the corticospinal tract. No established neuroimaging biomarker is associated with this condition.
Methods: A total of 46 patients affected by HSP, genetically and clinically evaluated and tested with SPRS scores, and 46 healthy controls (HC) matched by age and gender underwent a single-voxel Magnetic Resonance Spectroscopy sampling (MRS) of bilateral pre-central and pre-frontal regions. MRS data were analyzed cross-sectionally (at T 0 and T 1 ) and longitudinally (T 0 vs. T 1 ).
Results: Statistically significant data showed that T 0 mI/Cr in the pre-central areas of HSP patients was higher than in HC. In the left (L) pre-central area, NAA/Cr was significantly lower in HSP than in HC. In the right (R) pre-frontal area, NAA/Cr was significantly lower in HSP patients than in HC. HSP SPG4 subjects had significantly lower Cho/Cr concentrations in the L pre-central area compared to HC. Among the HSP subjects, non-SPG4 patients had significantly higher mI/Cr in the L pre-central area compared to SPG4 patients. In the R pre-frontal area, NAA/Cr was reduced, and ml/Cr was higher in non-SPG4 patients compared to SPG4 patients. Comparing "pure" and "complex" forms, NAA/Cr was higher in pHSP than in cHSP in the R pre-central and R pre-frontal areas. The longitudinal analysis, which involved fewer patients ( n  = 30), showed an increase in mI/Cr concentration in the L pre-frontal area among HSP subjects with respect to baseline. The patients had significantly higher SPRS scores at follow-up, with a significant positive correlation between SPRS scores and mI/Cr in the L pre-central area, while in bilateral pre-frontal areas, lower SPRS scores corresponded to higher NAA/Cr concentrations. To explore the discriminating power of MRS in correctly identifying HSP and controls, an inference tree methodology classified HSP subjects and controls with an overall accuracy of 73.9%, a sensitivity of 87.0%, and a specificity of 60.9%.
Conclusion: This pilot study indicates that brain MRS is a valuable approach that could potentially serve as an objective biomarker in HSP.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.
(Copyright © 2024 Montanaro, Vavla, Frijia, Coi, Baratto, Pasquariello, Stefan and Martinuzzi.)
Databáze: MEDLINE
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