CRISPR/Cas12a and Hybridization Chain Reaction-Coregulated Magnetic Relaxation Switching Biosensor for Sensitive Detection of Viable Salmonella in Animal-Derived Foods.

Autor: Gu A; College of Food Science and Technology, Huazhong Agricultural University, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, China., Dong Y; State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian, Liaoning 116034, China.; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China., Li L; College of Food Science and Technology, Huazhong Agricultural University, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, China., Yu D; State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian, Liaoning 116034, China.; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China., Zhang J; School of Life Science, Beijing Institute of Technology, Beijing 100081, P. R. China., Chen Y; College of Food Science and Technology, Huazhong Agricultural University, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, China.; State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian, Liaoning 116034, China.; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China.
Jazyk: angličtina
Zdroj: Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Sep 11; Vol. 72 (36), pp. 20130-20139. Date of Electronic Publication: 2024 Aug 28.
DOI: 10.1021/acs.jafc.4c05540
Abstrakt: We combined a CRISPR/Cas12a system with a hybridization chain reaction (HCR) to develop an ultrasensitive magnetic relaxation switching (MRS) biosensor for detecting viable Salmonella typhimurium ( S. typhimurium ). Magnetic nanoparticles of two sizes (30 and 1000 nm: MNP 30 and MNP 1000 , respectively) were coupled through HCR. The S. typhimurium gene-activated CRISPR/Cas12a system released MNP 30 from the MNP 1000 -HCR-MNP 30 complex through a trans-cleavage reaction. After magnetic separation, released MNP 30 was collected from the supernatant and served as a transverse relaxation time (T 2 ) signal probe. Quantitative detection of S. typhimurium is achieved by establishing a linear relationship between the change in T 2 and the target gene. The biosensor's limit of detection was 77 CFU/mL (LOD = 3 S / M , S = 22.30, M = 0.87), and the linear range was 10 2 -10 8 CFU/mL. The accuracy for detecting S. typhimurium in real samples is comparable to that of qPCR. Thus, this is a promising method for the rapid and effective detection of foodborne pathogens.
Databáze: MEDLINE
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