Aspergillus fumigatus conidial surface-associated proteome reveals factors for fungal evasion and host immunity modulation.

Autor: Pinzan CF; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil., Valero C; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.; Manchester Fungal Infection Group, Division of Evolution, Infection, and Genomics, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK., de Castro PA; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil., da Silva JL; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil., Earle K; Manchester Fungal Infection Group, Division of Evolution, Infection, and Genomics, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK., Liu H; Division of Infectious Diseases, Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, USA., Horta MAC; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil., Kniemeyer O; Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (Leibniz-HKI) and Institute of Microbiology, Friedrich Schiller University, Jena, Germany., Krüger T; Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (Leibniz-HKI) and Institute of Microbiology, Friedrich Schiller University, Jena, Germany., Pschibul A; Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (Leibniz-HKI) and Institute of Microbiology, Friedrich Schiller University, Jena, Germany., Cömert DN; Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (Leibniz-HKI) and Institute of Microbiology, Friedrich Schiller University, Jena, Germany., Heinekamp T; Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (Leibniz-HKI) and Institute of Microbiology, Friedrich Schiller University, Jena, Germany., Brakhage AA; Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (Leibniz-HKI) and Institute of Microbiology, Friedrich Schiller University, Jena, Germany., Steenwyk JL; Howards Hughes Medical Institute and the Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Mead ME; Department of Biological Sciences and Evolutionary Studies Initiative, Vanderbilt University, Nashville, TN, USA., Hermsdorf N; Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (Leibniz-HKI) and Institute of Microbiology, Friedrich Schiller University, Jena, Germany., Filler SG; Division of Infectious Diseases, Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, USA.; David Geffen School of Medicine at UCLA, Los Angeles, CA, USA., da Rosa-Garzon NG; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil., Delbaje E; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil., Bromley MJ; Manchester Fungal Infection Group, Division of Evolution, Infection, and Genomics, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.; Antimicrobial Resistance Network, University of Manchester, Manchester, UK., Cabral H; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil., Diehl C; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil., Angeli CB; Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil., Palmisano G; Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil., Ibrahim AS; Division of Infectious Diseases, Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, USA.; David Geffen School of Medicine at UCLA, Los Angeles, CA, USA., Rinker DC; Department of Biological Sciences and Evolutionary Studies Initiative, Vanderbilt University, Nashville, TN, USA., Sauters TJC; Department of Biological Sciences and Evolutionary Studies Initiative, Vanderbilt University, Nashville, TN, USA., Steffen K; Department of Biological Sciences and Evolutionary Studies Initiative, Vanderbilt University, Nashville, TN, USA., Gumilang A; Department of Biological Sciences and Evolutionary Studies Initiative, Vanderbilt University, Nashville, TN, USA., Rokas A; Department of Biological Sciences and Evolutionary Studies Initiative, Vanderbilt University, Nashville, TN, USA. antonis.rokas@vanderbilt.edu., Gago S; Manchester Fungal Infection Group, Division of Evolution, Infection, and Genomics, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK. sara.gago-2@manchester.ac.uk., Dos Reis TF; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil. thailaf@hotmail.com., Goldman GH; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil. ggoldman@usp.br.; National Institute of Science and Technology in Human Pathogenic Fungi, São Paulo, Brazil. ggoldman@usp.br.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2024 Oct; Vol. 9 (10), pp. 2710-2726. Date of Electronic Publication: 2024 Aug 27.
DOI: 10.1038/s41564-024-01782-y
Abstrakt: Aspergillus fumigatus causes aspergillosis and relies on asexual spores (conidia) for initiating host infection. There is scarce information about A. fumigatus proteins involved in fungal evasion and host immunity modulation. Here we analysed the conidial surface proteome of A. fumigatus, two closely related non-pathogenic species, Aspergillus fischeri and Aspergillus oerlinghausenensis, as well as pathogenic Aspergillus lentulus, to identify such proteins. After identifying 62 proteins exclusively detected on the A. fumigatus conidial surface, we assessed null mutants for 42 genes encoding these proteins. Deletion of 33 of these genes altered susceptibility to macrophage, epithelial cells and cytokine production. Notably, a gene that encodes a putative glycosylasparaginase, modulating levels of the host proinflammatory cytokine IL-1β, is important for infection in an immunocompetent murine model of fungal disease. These results suggest that A. fumigatus conidial surface proteins are important for evasion and modulation of the immune response at the onset of fungal infection.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
Externí odkaz: