Transposon mutagenesis screen in Klebsiella pneumoniae identifies genetic determinants required for growth in human urine and serum.

Autor:	Gray J; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom.; Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia., Torres VVL; Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia., Goodall E; Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia., McKeand SA; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Scales D; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Collins C; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Wetherall L; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Lian ZJ; Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia., Bryant JA; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Milner MT; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Dunne KA; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Icke C; Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia., Rooke JL; Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia., Schneiders T; Division of Infection Medicine, University of Edinburgh, Edinburgh, United Kingdom., Lund PA; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Cunningham AF; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, United Kingdom., Cole JA; Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom., Henderson IR; Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2024 Aug 27; Vol. 12. Date of Electronic Publication: 2024 Aug 27.
DOI:	10.7554/eLife.88971
Abstrakt:	Klebsiella pneumoniae is a global public health concern due to the rising myriad of hypervirulent and multidrug-resistant clones both alarmingly associated with high mortality. The molecular mechanisms underpinning these recalcitrant K. pneumoniae infection, and how virulence is coupled with the emergence of lineages resistant to nearly all present-day clinically important antimicrobials, are unclear. In this study, we performed a genome-wide screen in K. pneumoniae ECL8, a member of the endemic K2-ST375 pathotype most often reported in Asia, to define genes essential for growth in a nutrient-rich laboratory medium (Luria-Bertani [LB] medium), human urine, and serum. Through transposon directed insertion-site sequencing (TraDIS), a total of 427 genes were identified as essential for growth on LB agar, whereas transposon insertions in 11 and 144 genes decreased fitness for growth in either urine or serum, respectively. These studies not only provide further knowledge on the genetics of this pathogen but also provide a strong impetus for discovering new antimicrobial targets to improve current therapeutic options for K. pneumoniae infections. Competing Interests: JG, VT, EG, SM, DS, CC, LW, ZL, JB, MM, KD, CI, JR, TS, PL, AC, JC, IH No competing interests declared (© 2023, Gray, Torres et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu