Quantitative analysis of selected alkaloids of Mitragyna speciosa using 1 H quantitative nuclear magnetic resonance spectroscopy.

Autor: Garba SA; Natural Medicines and Products Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia.; Chemistry Department, Faculty of Natural and Applied Sciences, Sule Lamido University, Kafin Hausa, Nigeria., Shaari K; Natural Medicines and Products Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia., Abdul Manap MR; Department of Chemistry, Faculty of Sciences, Universiti Putra Malaysia, Serdang, Malaysia., Lee SY; School of Food Studies & Gastronomy, Faculty of Social Sciences & Leisure Management, Taylor's University, Subang Jaya, Malaysia.; Food Security & Nutrition Impact Lab, Taylor's University, Subang Jaya, Selangor, Malaysia., Abdulazeez I; Chemistry Department, School of Secondary Education Sciences, Federal College of Education Zaria, Tudun Wada, Nigeria., Mohd Faudzi SM; Natural Medicines and Products Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia.; Department of Chemistry, Faculty of Sciences, Universiti Putra Malaysia, Serdang, Malaysia.
Jazyk: angličtina
Zdroj: Magnetic resonance in chemistry : MRC [Magn Reson Chem] 2024 Nov; Vol. 62 (11), pp. 803-813. Date of Electronic Publication: 2024 Aug 27.
DOI: 10.1002/mrc.5477
Abstrakt: Mitragyna speciosa is a perennial plant native to Asia, well known for its psychoactive properties. Its major alkaloid mitragynine is known to have sedative and euphoric effects. Hence, the plant has been a subject of abuse, leading to addiction, necessitating efficient analytical methods to detect its psychoactive constituents. However, current chromatography-based methods for detecting the alkaloids are time consuming and costly. Quantitative nuclear magnetic resonance (qNMR) spectroscopy emerges as a promising alternative due to its nondestructive nature, structural insights, and short analysis time. Hence, a rapid and precise qNMR method was developed to quantify selected major psychoactive alkaloids in various parts of M. speciosa. Mitragynine, specioliatine, and speciogynine were quantified in relation to the integral value of the -OCH 3 groups of the alkaloids and the internal standard 1,4-dinitrobenzene. The precision and reproducibility of the method gave a relative standard deviation (RSD) of 2%, demonstrating the reliability of the method. In addition, the method showed excellent specificity, sensitivity, high linearity range (R 2  = 0.999), and limits of detection (LOD) and quantification (LOQ) values. The analysis revealed that the red-veined M. speciosa leaves contained higher levels of mitragynine (32.34 mg/g), specioliatine (16.84 mg/g) and speciogynine (7.69 mg/g) compared to the green-veined leaves, stem bark, or fruits.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: