Evaluating the coral microbiome during cryopreservation.

Autor: Jefferson T; University of North Carolina Wilmington, 601 S. College Road, Wilmington, NC, 28403, USA., Henley EM; Smithsonian National Zoo and Conservation Biology Institute, Front Royal, VA, 22360, USA; Hawaii Institute of Marine Biology, Kāne'ohe, HI, 96744, USA., Erwin PM; University of North Carolina Wilmington, 601 S. College Road, Wilmington, NC, 28403, USA; Center for Marine Science, 5600 Marvin K. Moss Lane, Wilmington, NC, 28409, USA., Lager C; Smithsonian National Zoo and Conservation Biology Institute, Front Royal, VA, 22360, USA; Hawaii Institute of Marine Biology, Kāne'ohe, HI, 96744, USA., Perry R; Smithsonian National Zoo and Conservation Biology Institute, Front Royal, VA, 22360, USA; Hawaii Institute of Marine Biology, Kāne'ohe, HI, 96744, USA., Chernikhova D; Environment and Natural Resources Program, Faculty of Life Sciences, University of Iceland, Reykjavík, Iceland., Powell-Palm MJ; J. Mike Walker '66 Department of Mechanical Engineering, Texas A&M University, College Station, TX, 77843, USA; Department of Materials Science and Engineering, Texas A&M University, College Station, TX, 77843, USA., Ushijima B; University of North Carolina Wilmington, 601 S. College Road, Wilmington, NC, 28403, USA., Hagedorn M; Smithsonian National Zoo and Conservation Biology Institute, Front Royal, VA, 22360, USA; Hawaii Institute of Marine Biology, Kāne'ohe, HI, 96744, USA. Electronic address: hagedornm@si.edu.
Jazyk: angličtina
Zdroj: Cryobiology [Cryobiology] 2024 Sep 17; Vol. 117, pp. 104960. Date of Electronic Publication: 2024 Sep 17.
DOI: 10.1016/j.cryobiol.2024.104960
Abstrakt: Coral reefs are threatened by various local and global stressors, including elevated ocean temperatures due to anthropogenic climate change. Coral cryopreservation could help secure the diversity of threatened corals. Recently, isochoric vitrification was used to demonstrate that coral fragments lived to 24 hr post-thaw; however, in this study, they were stressed post-thaw. The microbial portion of the coral holobiont has been shown to affect host fitness and the impact of cryopreservation treatment on coral microbiomes is unknown. Therefore, we examined the coral-associated bacterial communities pre- and post-cryopreservation treatments, with a view towards informing potential future stress reduction strategies. We characterized the microbiome of the Hawaiian finger coral, Porites compressa in the wild and at seven steps during the isochoric vitrification process. We observed significant changes in microbiome composition, including: 1) the natural wild microbiomes of P. compressa were dominated by Endozoicomonadaceae (76.5 % relative abundance) and consistent between samples, independent of collection location across Kāne'ohe Bay; 2) Endozoicomonadaceae were reduced to <6.9 % in captivity, and further reduced to <0.5 % relative abundance after isochoric vitrification; and 3) Vibrionaceae dominated communities post-thaw (58.5-74.7 % abundance). Thus, the capture and cryopreservation processes, are implicated as possible causal agents of dysbiosis characterized by the loss of putatively beneficial symbionts (Endozoicomonadaceae) and overgrowth of potential pathogens (Vibrionaceae). Offsetting these changes with probiotic restoration treatments may alleviate cryopreservation stress and improve post-thaw husbandry.
Competing Interests: Declaration of competing interest On behalf of all authors, the corresponding author states that there is no conflict of interest.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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