Chemiluminescent/photothermal dual-mode lateral flow immunoassay based on CoFe PBAs/WS 2 nanozyme for rapid and highly sensitive point-of-care testing of gentamicin.

Autor: Wu L; Department of Pharmacy, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, China., Zhu Z; Department of Pharmacy, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, China., Xue J; The State Key Lab of Resource Insects, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China., Zheng L; Department of Pharmacy, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, China., Liu H; Department of Pharmacy, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, China., Ouyang H; The State Key Lab of Resource Insects, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China., Fu Z; The State Key Lab of Resource Insects, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China. Electronic address: fuzf@swu.edu.cn., He Y; Department of Pharmacy, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, China. Electronic address: heyongzmu@163.com.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2024 Dec 01; Vol. 265, pp. 116711. Date of Electronic Publication: 2024 Aug 24.
DOI: 10.1016/j.bios.2024.116711
Abstrakt: Serious adverse drug reactions of gentamicin (GM) significantly limit its clinical use, thus there is an urgent demand to develop reliable strategies to detect its concentration. In this study, we have developed a novel highly sensitive and portable lateral flow immunoassay (LFIA) based on CoFe PBAs/WS 2 nanozyme mediated chemiluminescence (CL) and photothermal (PT) dual-mode POCT biosensor for the detection of GM, which successfully combines sensitive laboratory analyses with portable in situ analyses in the field. In this proof-of-principle work, the dynamic detection ranges of CL-LFIA and PT-LFIA mode were 1 pg mL -1 to 100 ng mL -1 and 50 pg mL -1 to 100 ng mL -1 with the limits of detection of 0.33 and 16.67 pg mL -1 , respectively. The whole detection of CL-LFIA and PT-LFIA could be completed within 15 min and 30 min, respectively. The recoveries of GM spiked into complex matrices including milk, urine, and serum for CL-LFIA and PT-LFIA were 90.94%-109.74% and 94.49%-109.31%, respectively, indicating the reliability and applicability of the dual-mode LFIA in real samples. The dual-mode POCT biosensor could effectively overcome the false problems with improving accuracy and sensitivity, enabling user to precisely detect GM by laboratory analysis or on-site analysis depending on the source condition. Due to the complementary properties of CL-LFIA and PT-LFIA, the developed POCT biosensor can effectively ensure high-performance detection, showing the potential application of accurately detecting drug concentration in clinical practice.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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