Prevotella are major contributors of sialidases in the human vaginal microbiome.

Autor:	Pelayo P; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138., Hussain FA; Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard, Massachusetts General Hospital, Cambridge, MA 02139., Werlang CA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139., Wu CM; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139., Woolston BM; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138.; Department of Chemical Engineering, Northeastern University, Boston, MA 02115., Xiang CM; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138., Rutt L; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201., France MT; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201., Ravel J; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201., Ribbeck K; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139., Kwon DS; Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard, Massachusetts General Hospital, Cambridge, MA 02139., Balskus EP; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138.; HHMI, Harvard University, Cambridge, MA 02138.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Sep 03; Vol. 121 (36), pp. e2400341121. Date of Electronic Publication: 2024 Aug 26.
DOI:	10.1073/pnas.2400341121
Abstrakt:	Elevated bacterial sialidase activity in the female genital tract is strongly associated with poor health outcomes including preterm birth and bacterial vaginosis (BV). These negative effects may arise from sialidase-mediated degradation of the protective mucus layer in the cervicovaginal environment. Prior biochemical studies of vaginal bacterial sialidases have focused solely on the BV-associated organism Gardnerella vaginalis . Despite their implications for sexual and reproductive health, sialidases from other vaginal bacteria have not been characterized. Here, we show that vaginal Prevotella species produce sialidases that possess variable activity toward mucin substrates. The sequences of sialidase genes and their presence are largely conserved across clades of Prevotella from different geographies, hinting at their importance globally. Finally, we find that Prevotella sialidase genes and transcripts, including those encoding mucin-degrading sialidases from Prevotella timonensis , are highly prevalent and abundant in human vaginal genomes and transcriptomes. Together, our results identify Prevotella as a critical source of sialidases in the vaginal microbiome, improving our understanding of this detrimental bacterial activity. Competing Interests: Competing interests statement:J.R. is a cofounder of LUCA Biologics, a biotechnology company focusing on translating microbiome research into live biotherapeutic drugs for women’s health. D.S.K. serves as equity holder of Day Zero Diagnostics.
Databáze:	MEDLINE
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