Novel tau filament folds in individuals with MAPT mutations P301L and P301T.

Autor:	Schweighauser M; MRC Laboratory of Molecular Biology, Cambridge, UK.; These authors contributed equally., Shi Y; MRC Laboratory of Molecular Biology, Cambridge, UK.; Department of Pathology of the First Affiliated Hospital and School of Brain Science, Zhejiang University, Hangzhou, China.; These authors contributed equally., Murzin AG; MRC Laboratory of Molecular Biology, Cambridge, UK.; These authors contributed equally., Garringer HJ; Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, USA., Vidal R; Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, USA., Murrell JR; Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, USA.; Department of Pathology and Laboratory Medicine, Children's Hospital of the University of Pennsylvania, Philadelphia, USA., Erro ME; Department of Neurology, Hospital Universitario de Navarra, Brain Bank NavarraBiomed, Pamplona, Spain., Seelaar H; Department of Neurology, Erasmus University, Rotterdam, The Netherlands., Ferrer I; Department of Pathology and Experimental Therapeutics, University of Barcelona, Spain., van Swieten JC; Department of Neurology, Erasmus University, Rotterdam, The Netherlands., Ghetti B; Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, USA., Scheres SHW; MRC Laboratory of Molecular Biology, Cambridge, UK.; These authors jointly supervised the work., Goedert M; MRC Laboratory of Molecular Biology, Cambridge, UK.; These authors jointly supervised the work.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Aug 17. Date of Electronic Publication: 2024 Aug 17.
DOI:	10.1101/2024.08.15.608062
Abstrakt:	Mutations in MAPT , the microtubule-associated protein tau gene, give rise to cases of frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17) with abundant filamentous tau inclusions in brain cells. Individuals with pathological MAPT variants exhibit behavioural changes, cognitive impairment and signs of parkinsonism. Missense mutations of residue P301, which are the most common MAPT mutations associated with FTDP-17, give rise to the assembly of mutant four-repeat tau into filamentous inclusions, in the absence of extracellular deposits. Here we report the cryo-EM structures of tau filaments from five individuals belonging to three unrelated families with mutation P301L and from one individual belonging to a family with mutation P301T. A novel three-lobed tau fold resembling the two-layered tau fold of Pick's disease was present in all cases with the P301L tau mutation. Two different tau folds were found in the case with mutation P301T, the less abundant of which was a variant of the three-lobed fold. The major P301T tau fold was V-shaped, with partial similarity to the four-layered tau folds of corticobasal degeneration and argyrophilic grain disease. These findings suggest that FTDP-17 with mutations in P301 should be considered distinct inherited tauopathies and that model systems with these mutations should be used with caution in the study of sporadic tauopathies. Competing Interests: Competing interests The authors have no competing interests.
Databáze:	MEDLINE
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