Imaging high-frequency voltage dynamics in multiple neuron classes of behaving mammals.

Autor:	Haziza S; James H. Clark Center, Stanford University, Stanford, CA 94305, USA.; CNC Program, Stanford University, Stanford, CA 94305, USA., Chrapkiewicz R; James H. Clark Center, Stanford University, Stanford, CA 94305, USA.; CNC Program, Stanford University, Stanford, CA 94305, USA., Zhang Y; James H. Clark Center, Stanford University, Stanford, CA 94305, USA.; CNC Program, Stanford University, Stanford, CA 94305, USA.; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA., Kruzhilin V; James H. Clark Center, Stanford University, Stanford, CA 94305, USA.; CNC Program, Stanford University, Stanford, CA 94305, USA., Li J; James H. Clark Center, Stanford University, Stanford, CA 94305, USA.; CNC Program, Stanford University, Stanford, CA 94305, USA., Li J; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA., Delamare G; CNC Program, Stanford University, Stanford, CA 94305, USA., Swanson R; Neuroscience Institute, Langone Medical Center, New York University, New York, NY 10016, USA., Buzsáki G; Neuroscience Institute, Langone Medical Center, New York University, New York, NY 10016, USA.; Department of Neurology, Langone Medical Center, New York University, New York, NY 10016, USA., Kannan M; Department of Neuroscience, University of Minnesota, Minneapolis, MN 55455, USA., Vasan G; Department of Neuroscience, University of Minnesota, Minneapolis, MN 55455, USA., Lin MZ; Departments of Bioengineering & Pediatrics, Stanford University, Stanford CA 94305, USA., Zeng H; Allen Institute for Brain Science, Seattle, WA 98109, USA., Daigle TL; Allen Institute for Brain Science, Seattle, WA 98109, USA., Schnitzer MJ; James H. Clark Center, Stanford University, Stanford, CA 94305, USA.; CNC Program, Stanford University, Stanford, CA 94305, USA.; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.; Lead contact.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Aug 16. Date of Electronic Publication: 2024 Aug 16.
DOI:	10.1101/2024.08.15.607428
Abstrakt:	Fluorescent genetically encoded voltage indicators report transmembrane potentials of targeted cell-types. However, voltage-imaging instrumentation has lacked the sensitivity to track spontaneous or evoked high-frequency voltage oscillations in neural populations. Here we describe two complementary TEMPO voltage-sensing technologies that capture neural oscillations up to ~100 Hz. Fiber-optic TEMPO achieves ~10-fold greater sensitivity than prior photometry systems, allows hour-long recordings, and monitors two neuron-classes per fiber-optic probe in freely moving mice. With it, we uncovered cross-frequency-coupled theta- and gamma-range oscillations and characterized excitatory-inhibitory neural dynamics during hippocampal ripples and visual cortical processing. The TEMPO mesoscope images voltage activity in two cell-classes across a ~8-mm-wide field-of-view in head-fixed animals. In awake mice, it revealed sensory-evoked excitatory-inhibitory neural interactions and traveling gamma and 3-7 Hz waves in the visual cortex, and previously unreported propagation directions for hippocampal theta and beta waves. These technologies have widespread applications probing diverse oscillations and neuron-type interactions in healthy and diseased brains. Competing Interests: DECLARATION OF INTERESTS M.J.S. is a co-author of a U.S. patent covering technologies in this paper.
Databáze:	MEDLINE
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