The Association of MMP-2, MMP-9, and MMP-13 Gene Polymorphisms With Knee Osteoarthritis in the Greek Population.

Autor:	Milaras C; 4th Orthopedic Department, KAT Hospital, Athens, GRC., Lepetsos P; 4th Orthopedic Department, KAT Hospital, Athens, GRC., Pampanos A; Medical Genetics Department, 'Alexandra' General Hospital, Athens, GRC., Kenanidis E; Academic Orthopedic Department, Papageorgiou General Hospital, Aristotle University of Thessaloniki, School of Medicine, Thessaloniki, GRC.; Center of Orthopedic and Regenerative Medicine - Center of Interdisciplinary Research and Innovation, Aristotle University of Thessaloniki, School of Medicine, Thessaloniki, GRC., Macheras GA; 4th Orthopedic Department, KAT Hospital, Athens, GRC., Mavrogianni D; 1st Obstetrics and Gynecology Department, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, Medical School, Athens, GRC., Dafou D; Biology Department, Aristotle University of Thessaloniki, Thessaloniki, GRC.; Center of Orthopedic and Regenerative Medicine - Center of Interdisciplinary Research and Innovation, Aristotle University of Thessaloniki, School of Medicine, Thessaloniki, GRC., Potoupnis M; Academic Orthopedic Department, Papageorgiou General Hospital, Aristotle University of Thessaloniki, School of Medicine, Thessaloniki, GRC., Tsiridis E; Academic Orthopedic Department, Papageorgiou General Hospital, Aristotle University of Thessaloniki, School of Medicine, Thessaloniki, GRC.; Center of Orthopedic and Regenerative Medicine - Center of Interdisciplinary Research and Innovation, Aristotle University of Thessaloniki, School of Medicine, Thessaloniki, GRC.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2024 Jul 25; Vol. 16 (7), pp. e65379. Date of Electronic Publication: 2024 Jul 25 (Print Publication: 2024).
DOI:	10.7759/cureus.65379
Abstrakt:	Introduction Primary knee osteoarthritis (OA) is a multifactorial degenerative joint disorder characterized by articular cartilage degradation. Matrix metalloproteinases (MMPs) have been reported to play a vital role in OA pathogenesis, significantly contributing to extracellular matrix (ECM) catabolism. The purpose of this study is to investigate the association of MMP-2 -1575G/A (rs243866), MMP-9 836A/G (rs17576), and MMP-13 -77A/G (rs2252070) gene polymorphisms with knee OA in the Greek population. Methods One hundred patients (24% males, mean age: 68.3 years) with primary knee OA were included in the study along with 100 controls (47% males, mean age: 65.2 years). Genotypes were identified through polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) technique. Allelic and genotypic frequencies were compared between patients and controls. Results The MMP-13 -77A/G polymorphism was significantly associated with knee OA in the crude analysis (P = 0.008). After binary logistic regression analysis, the dominant model of the MMP-13-77A/G (AG + GG versus AA) was found to be associated with increased risk for knee OA (odds ratio (OR) = 2.290, 95% confidence interval (95%CI) = 1.059-4.949, P= 0.035). Compared to the A allele, the G allele in the MMP-13rs2252070 locus was a predictive factor for knee OA (OR = 2.351, 95%CI = 1.134-4.874, P= 0.022). No significant associations were detected for the MMP-2 -1575G/A and MMP-9 836A/G polymorphisms (P > 0.05). Conclusions The present study shows that the MMP-2 -1575G/A and MMP-9 836A/G polymorphisms are not significantly associated with primary knee OA in the Greek population. The MMP-13 -77A/G was found to be a significant risk factor for knee OA in the Greek population. Additional research is needed to verify this association in larger and different populations, in different joints, to elucidate the role of this single nucleotide polymorphism (SNP) in OA pathogenesis. Competing Interests: Human subjects: Consent was obtained or waived by all participants in this study. The Ethics Review Committee of Papageorgiou General Hospital issued approval 197/17.6.2020. The study was approved by the Ethics Review Committee of Papageorgiou General Hospital (197/17.6.2020). The hospital belongs to Aristotle University of Thessaloniki, School of Medicine, Thessaloniki, Greece. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. (Copyright © 2024, Milaras et al.)
Databáze:	MEDLINE
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