Molecular e serological detection of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in domestic dogs in Manaus city, Amazonas, Brazil.

Autor:	Nascimento de Campos HG; Programa de Pós-Graduação em Saúde Única, Faculdade de Medicina Veterinária, Universidade Santo Amaro, São Paulo, Brazil., Gennari SM; Programa de Pós-Graduação em Saúde Única, Faculdade de Medicina Veterinária, Universidade Santo Amaro, São Paulo, Brazil., da Silva RE; Faculdade de Medicina, Universidade Santo Amaro, São Paulo, Brazil., Soares HS; Programa de Pós-Graduação em Saúde Única, Faculdade de Medicina Veterinária, Universidade Santo Amaro, São Paulo, Brazil., Costa JOJ; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., de Azevedo SS; Unidade Acadêmica de Medicina Veterinária, Universidade Federal de Campina Grande, Patos, Brazil., Marcili A; Programa de Pós-Graduação em Saúde Única, Faculdade de Medicina Veterinária, Universidade Santo Amaro, São Paulo, Brazil.; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of medical entomology [J Med Entomol] 2024 Aug 25. Date of Electronic Publication: 2024 Aug 25.
DOI:	10.1093/jme/tjae110
Abstrakt:	Leishmaniasis are zoonosis widely spread in Brazil, caused by the protozoan of the genus Leishmania, which includes several species. The disease manifests itself in the visceral or tegumentary form, and the main reservoir is the dogs. Manaus is the largest city in the Brazilian Amazon region, and despite the importance of the municipality, practically nothing is known about leishmaniosis in humans and animals. The objective of this study was to evaluate the occurrence and risk factors associated with the presence of Leishmania infantum in domiciliated dogs from Manaus. Molecular (polymerase chain reaction) and serological (immunofluorescent antibody test) methods were used as an indication of the circulation of the parasite. Blood samples for 154 domiciled dogs were obtained, and prevalence ratio and analysis of the variables were performed. Serum antibodies anti-Leishmania spp. were detected in 20.8% (95% CI: 14.4%-27.2%). Access to the street and zone of residence (P < 0.01) were associated with higher seropositivity. Molecular diagnosis for L. infantum detected positivity in 60 (39%) of the 154 (95% CI: 31.3%-46.7%) animals, and the variables street access, contact with dogs, and zone of residence were associated with higher frequencies of positivity (P < 0.05). Both serology and molecular diagnosis detected positive dogs in the municipality. This is the first description of the circulation of L. infantum infecting dogs in Manaus. As the municipality is classified as nonendemic, studies of isolation and characterization of the isolate must be done urgently. (© The Author(s) 2024. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
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