Impact of equine herpesvirus-1 ORF15 (EUL45) on viral replication and neurovirulence.

Autor: Kasem S; Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Elgeish Street, Kafr El Sheikh 33516, Egypt. Electronic address: samykasem2@gmail.com., Yu MHH; Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan., Alkhalefa N; Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Elgeish Street, Kafr El Sheikh 33516, Egypt., Ata EB; Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre (NRC), Egypt., Nayel M; Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City University, Egypt., Abdo W; Department of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Elgeish Street, Kafr El Sheikh 33516, Egypt., Abdel-Moneim AS; Department of Microbiology, College of Medicine, Taif University, Al-Taif 21944, Saudi Arabia. Electronic address: asa@tu.edu.sa., Fukushi H; Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2024 Nov; Vol. 298, pp. 110234. Date of Electronic Publication: 2024 Aug 22.
DOI: 10.1016/j.vetmic.2024.110234
Abstrakt: Equine herpesvirus 1 (EHV-1) causes respiratory illness, fetal loss, perinatal mortality, and myeloencephalopathy. This study investigated ORF15's impact on virus infectivity and neurovirulence. The Ab4p neurovirulent strain of EHV1 was used as a backbone to create Ab4p attB, Ab4p∆ORF15, and Ab4p∆ORF15R chimeras via BAC DNA transfection into RK-13 cells. Viral growth kinetics, plaque size, transcription, and growth were assessed in MDBK cells, mouse neurons, and fetal equine brain cells. Neurovirulence was evaluated post-intranasal inoculation into male CBA/N1 SPF mice, measuring signs, virus titers, and histopathological changes. Deletion of EUL45 (Ab4p-∆EUL45) reduced viral replication efficiency, resulting in decreased release and smaller plaques. EUL45 deletion also upregulated neighbouring genes (EUL46 and EUL44). Ab4p-∆EUL45 exhibited reduced virulence and poor growth in neural cells compared to wild-type viruses. This study sheds light on EUL45's role in EHV-1, viral replication, and regulation of EUL46 and EUL44 expression, suggesting potential as a vaccine candidate.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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