Hypericin-loaded in modified theranostic liposome nanoplatform: a preliminary in vivo study of targeting and diagnosis.

Autor: de Morais FAP; Technological Innovation Laboratory in Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá, PR, 87020-900, Brazil. flaviaapmorais@hotmail.com., Balbinot RB; Technological Innovation Laboratory in Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá, PR, 87020-900, Brazil., Bakoshi ABK; Technological Innovation Laboratory in Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá, PR, 87020-900, Brazil., Lararin-Bidoia D; Technological Innovation Laboratory in Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá, PR, 87020-900, Brazil., da Silva Souza Campanholi K; Department of Chemistry, State University of Maringá, Maringá, PR, 87020-900, Brazil., da Silva RC Jr; Department of Chemistry, State University of Maringá, Maringá, PR, 87020-900, Brazil., de França BM; Department of Chemistry, Federal University of Rio de Janeiro, 149 Athos da Silveira Ramos Ave., Rio de Janeiro, RJ, 21941-909, Brazil., Gonçalves RS; Laboratory of Chemistry of Natural Products, Department of Chemistry, Center of Exact Sciences and Technology, Federal University of Maranhão, São Luís, MA, 65080-805, Brazil., Ueda-Nakamura T; Technological Innovation Laboratory in Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá, PR, 87020-900, Brazil., de Oliveira Silva S; Technological Innovation Laboratory in Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá, PR, 87020-900, Brazil., Caetano W; Department of Chemistry, State University of Maringá, Maringá, PR, 87020-900, Brazil., Nakamura CV; Technological Innovation Laboratory in Pharmaceuticals and Cosmetics Development, State University of Maringá, Maringá, PR, 87020-900, Brazil.
Jazyk: angličtina
Zdroj: Naunyn-Schmiedeberg's archives of pharmacology [Naunyn Schmiedebergs Arch Pharmacol] 2024 Aug 24. Date of Electronic Publication: 2024 Aug 24.
DOI: 10.1007/s00210-024-03379-y
Abstrakt: Modified theranostic liposomes were created by combining phospholipid 1,2-dipalmitoyl-sn-3-glycerol-phosphatidylcholine with two previously modified Pluronic ® copolymers covalently linked with spermine and folic acid to carry and stabilize the photosensitizer compound hypericin. After physicochemical characterization, the photocytotoxicity was evaluated against different cancer and healthy cells presenting a strong photodynamic effect. The formulation exhibited no photoactivity without illumination and without hypericin. In vivo, pharmacokinetics biodistribution examined the uptake and theranostic potential of this nanoformulation after its intravenous administration in animal models. Fluorescence images revealed the maximum fluorescence between 0.5-4 h post-tail vein injection, making it an appropriate period for photodynamic treatment. The fluorescence of the entire body was monitored for at least 3 days, indicating that the theranostic procedures can be performed within the 0.5-4 h range after administration, after which the intensity decreases, indicating a potent metabolic ability with no significant side effects. The fluorescence images of the main organs consistently showed a signal during the 1st day of its application. After 48 h, only residues of the modified theranostic formulation were detected in the lungs and thyroid. The promising pharmacokinetics observed in our preliminary studies highlight the potential of this system, making it a worthy candidate for further investigation with tumor models.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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