Biomimetic fiber/hydrogel composite scaffolds based on chitosan hydrogel and surface modified PCL chopped-microfibers.

Autor: Jamali SA; Department of Polymer Engineering, Qom University of Technology, Qom, Iran., Mohammadi M; Department of Polymer Engineering, Qom University of Technology, Qom, Iran. Electronic address: mohammadi@qut.ac.ir., Saeed M; Soft Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Central Tehran Branch, Islamic Azad University, Tehran, Iran., Haramshahi SMA; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran., Shahmahmoudi Z; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran., Pezeshki-Modaress M; Burn Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Plastic and Reconstructive Surgery, Hazrat Fatemeh Hospital, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address: Pezeshkimodaress.m@iums.ac.ir.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Oct; Vol. 278 (Pt 3), pp. 134936. Date of Electronic Publication: 2024 Aug 22.
DOI: 10.1016/j.ijbiomac.2024.134936
Abstrakt: Hydrogel/fiber composites have received wide attention as tissue engineering scaffolds due to the outstanding properties of fibers and hydrogels. In the current research, a hydrogel/fiber composite scaffold was made based on chitosan-modified polycaprolactone (PCL) microfibers and chitosan hydrogel as a binder. The presence of chitosan as a modifier on the surface of fibers and as a binder between fibers can create scaffolds with excellent structural and mechanical properties. To this end, the three-dimensional microfibers were first functionalized with amine groups. Then, the chitosan chains were attached to the fibers by an aldehyde coupling agent and Schiff base reaction. FTIR and Raman spectroscopies corroborated that chitosan was successfully immobilized on PCL fibers. Chitosan-modified fibers were molded with chitosan solutions of various concentrations and the prepared composite scaffolds were stabilized using ionic crosslinking. The obtained composites represented a porous 3D structure with highly interconnected pores. The compressive modulus increased by 19 and 2.7 folds and the tensile modulus was augmented by 28 and 4 folds, in respective dry and swollen states with increasing hydrogel concentration from 0.1 to 1 %. Hydrogel/fiber composites were able to preserve cell viability, and increasing the hydrogel proportion increased adhesion, proliferation and penetration of cells into the scaffold.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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