Use of waste frying oil and coconut pulp for the production, isolation, and characterization of a new lipase from Moesziomyces aphidis.

Autor: Ferreira AN; Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), 57072-900, Maceió, Alagoas, Brazil., Silva TP; Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), 57072-900, Maceió, Alagoas, Brazil., Félix CR; Institute of Biological and Health Sciences, Federal University of Alagoas, A. C. Simões Campus, (UFAL), 57072-900, Maceió, Alagoas, Brazil., Lopes JL; Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), 57072-900, Maceió, Alagoas, Brazil., Dos Santos CWV; Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), 57072-900, Maceió, Alagoas, Brazil., Dos Santos DMRC; Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), 57072-900, Maceió, Alagoas, Brazil., Landell MF; Institute of Biological and Health Sciences, Federal University of Alagoas, A. C. Simões Campus, (UFAL), 57072-900, Maceió, Alagoas, Brazil., Gomes FS; Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), 57072-900, Maceió, Alagoas, Brazil., Pereira HJV; Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), 57072-900, Maceió, Alagoas, Brazil. Electronic address: hugo.vieira@iqb.ufal.br.
Jazyk: angličtina
Zdroj: Protein expression and purification [Protein Expr Purif] 2025 Jan; Vol. 225, pp. 106584. Date of Electronic Publication: 2024 Aug 22.
DOI: 10.1016/j.pep.2024.106584
Abstrakt: Lipases comprise the third most commercialized group of enzymes worldwide and those of microbial origin are sought for their multiple advantages. Agro-industrial waste can be an alternative culture medium for producing lipases, reducing production costs and the improper disposal of waste frying oil (WFO). This study aimed to produce yeast lipases through submerged fermentation (SF) using domestic edible oil waste as inducer and alternative culture medium. The optimal culture conditions, most effective inducer, and purification method for a new lipase from Moesziomyces aphidis BRT57 were identified. Yeast was cultured in medium containing green coconut pulp and WFO waste for 72 h. The maximum production of lipases in SF occurred in a culture medium containing WFO and yeast extract at 48 and 72 h of incubation, with enzyme activities of 8.88 and 11.39 U mL -1 , respectively. The lipase was isolated through ultrafiltration followed by size exclusion chromatography, achieving a 50.46 % recovery rate. To the best of our knowledge, this is the first study to report the production and purification of lipases from M. aphidis, demonstrating the value of frying oil as inducer and alternative medium for SF, contributing to the production of fatty acids for biodiesel from food waste.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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