Mitochondrial free radicals contribute to cigarette smoke condensate-induced impairment of oxidative phosphorylation in the skeletal muscle in situ.
Autor: | Bannon ST; Department of Kinesiology, University of Massachusetts Amherst, MA, USA., Decker ST; Department of Kinesiology, University of Massachusetts Amherst, MA, USA; Diabetes and Metabolism Research Center, University of Utah, UT, USA., Erol ME; Department of Kinesiology, University of Massachusetts Amherst, MA, USA; School of Health and Kinesiology, University of Nebraska Omaha, NE, USA., Fan R; Department of Nutrition, University of Massachusetts Amherst, MA, USA., Huang YT; Department of Kinesiology, University of Massachusetts Amherst, MA, USA., Chung S; Department of Nutrition, University of Massachusetts Amherst, MA, USA., Layec G; Department of Kinesiology, University of Massachusetts Amherst, MA, USA; School of Health and Kinesiology, University of Nebraska Omaha, NE, USA. Electronic address: glayec@unomaha.edu. |
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Jazyk: | angličtina |
Zdroj: | Free radical biology & medicine [Free Radic Biol Med] 2024 Aug 22; Vol. 224, pp. 325-334. Date of Electronic Publication: 2024 Aug 22. |
DOI: | 10.1016/j.freeradbiomed.2024.08.024 |
Abstrakt: | Oxidative stress plays a critical role in cellular dysfunction associated with cigarette smoke exposure and aging. Some chemicals from tobacco smoke have the potential to amplify mitochondrial ROS (mROS) production, which, in turn, may impair mitochondrial respiratory function. Accordingly, the present study tested the hypothesis that a mitochondria-targeted antioxidant (MitoTEMPO, MT) would attenuate the inhibitory effects of cigarette smoke on skeletal muscle respiratory capacity of middle-aged mice. Specifically, mitochondrial oxidative phosphorylation was assessed using high-resolution respirometry in permeabilized fibers from the fast-twitch gastrocnemius muscle of middle-aged C57Bl/6J mice. Before the assessment of respiration, tissues were incubated for 1hr with a control buffer (CON), cigarette smoke condensate (2 % dilution, SMOKE), or MitoTEMPO (10 μM) combined with cigarette smoke condensate (MT + SMOKE). Cigarette smoke condensate (CSC) decreased maximal-ADP stimulated respiration (CON: 60 ± 15 pmolO (Copyright © 2024 Elsevier Inc. All rights reserved.) |
Databáze: | MEDLINE |
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