A σC-protein-based indirect enzyme-linked immunosorbent assay for clinical detection of antiavian reovirus antibodies.

Autor:	Yang X; National Key Laboratory of Veterinary Public Health Security, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Gao H; National Key Laboratory of Veterinary Public Health Security, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Cheng Z; National Key Laboratory of Veterinary Public Health Security, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Zhang S; National Key Laboratory of Veterinary Public Health Security, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Zhao Y; National Key Laboratory of Veterinary Public Health Security, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Zheng H; National Key Laboratory of Veterinary Public Health Security, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Gao L; National Key Laboratory of Veterinary Public Health Security, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Cao H; National Key Laboratory of Veterinary Public Health Security, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Li X; National Key Laboratory of Veterinary Public Health Security, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Zheng SJ; National Key Laboratory of Veterinary Public Health Security, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Wang Y; National Key Laboratory of Veterinary Public Health Security, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: vetwyq@cau.edu.cn.
Jazyk:	angličtina
Zdroj:	Poultry science [Poult Sci] 2024 Nov; Vol. 103 (11), pp. 104188. Date of Electronic Publication: 2024 Aug 08.
DOI:	10.1016/j.psj.2024.104188
Abstrakt:	Avian reovirus (ARV) is the causative agent of avian viral arthritis and causes significant economic losses to the global poultry industry. For clinical diagnosis, detecting ARV-specific antibodies is crucial. We successfully expressed the ARV-σC protein in insect cells using the baculovirus expression vector system, achieving an expression level of approximately 200 mg/L. We developed an indirect enzyme-linked immunosorbent assay (iELISA) using the ARV-σC protein as a coating antigen to detect antibodies against it. The inter-batch and intrabatch coefficients of iELISA variation were less than 10%. Its sensitivity (1:12,800 diluted in serum) was 4 times higher than that of the indirect immunofluorescence assay (IFA; 1:3200 diluted in serum), and it showed no cross-reactivity with antibodies against other common avian viruses (such as Infectious bursal disease virus, Newcastle disease virus). The practicality of the iELISA was further evaluated using clinical samples. 300 clinical sera from chickens vaccinated with the ARV attenuated vaccine and 20 SPF sera were tested using both the iELISA and the IFA, demonstrating a 100% conformity rate. In conclusion, these results suggest that the iELISA developed in this study is a rapid, sensitive, and specific method that could serve as an effective diagnostic tool for monitoring and controlling avian viral arthritis. Competing Interests: DISCLOSURES All authors disclosed no relevant relationships. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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