A complete 3D-printed tool kit for Solid-State NMR sample and rotor handling.

Autor: Olson MA; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706 USA., Han R; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706 USA., Ravula T; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706 USA; National Magnetic Resonance Facility at Madison (NMRFAM), University of Wisconsin-Madison, Madison, WI, 53706 USA., Borcik CG; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706 USA., Wang S; National Magnetic Resonance Facility at Madison (NMRFAM), University of Wisconsin-Madison, Madison, WI, 53706 USA., Viera PA; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706 USA; Biophysics Graduate Program, University of Wisconsin-Madison, Madison, WI, 53706 USA., Rienstra CM; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706 USA; Department of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706 USA; National Magnetic Resonance Facility at Madison (NMRFAM), University of Wisconsin-Madison, Madison, WI, 53706 USA. Electronic address: crienstra@wisc.edu.
Jazyk: angličtina
Zdroj: Journal of magnetic resonance (San Diego, Calif. : 1997) [J Magn Reson] 2024 Sep; Vol. 366, pp. 107748. Date of Electronic Publication: 2024 Aug 06.
DOI: 10.1016/j.jmr.2024.107748
Abstrakt: Solid state NMR (SSNMR) is a highly versatile and broadly applicable method for studying the structure and dynamics of biomolecules and materials. For scientists entering the field of SSNMR, the many quotidian activities required in the workflow to prepare samples for data collection can present a significant barrier to adoption. These steps include transfer of samples into rotors, marking the reflective surfaces for high sensitivity tachometer signal detection, inserting rotors into the magic-angle spinning (MAS) stator, achieving stable spinning, and removing and storing rotors to ensure reproducibility of data collection conditions. Even experienced spectroscopists experience occasional problems with these operations, and the cumulative probability of a delay to successful data collection is high enough to cause frequent disruptions to instrument schedules, particularly in the context of large facilities serving a diverse community of users. These problems are all amplified when utilizing rotors smaller than about 4 mm in diameter. Therefore, to improve the reliability and robustness of SSNMR sample preparation workflows, here we describe a set of tools for rotor packing, unpacking, tachometer marking, extraction and storage. Stereolithography 3D printing was employed as a cost-effective and convenient method for prototyping and manufacturing a full range of designs suitable for several types of probes and rotor geometries.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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