The Allium cepa comet assay for environmental sample assessment: a scoping review.

Autor:	Alias C; Department of Medical and Surgical Specialties, University of Brescia, Radiological Sciences and Public Health, 25128 Brescia, Italy., Zerbini I; Department of Medical and Surgical Specialties, University of Brescia, Radiological Sciences and Public Health, 25128 Brescia, Italy., Zani C; Department of Medical and Surgical Specialties, University of Brescia, Radiological Sciences and Public Health, 25128 Brescia, Italy., Feretti D; Department of Medical and Surgical Specialties, University of Brescia, Radiological Sciences and Public Health, 25128 Brescia, Italy.
Jazyk:	angličtina
Zdroj:	Mutagenesis [Mutagenesis] 2024 Sep 27; Vol. 39 (4-5), pp. 219-237.
DOI:	10.1093/mutage/geae020
Abstrakt:	The purposes of this review were to investigate the application of the comet assay in Allium cepa root cells to assess the genotoxicity of environmental samples and to analyse the experimental procedures employed. A literature search was performed selecting articles published between January 2000 and October 2023 from online databases using the combined search terms 'comet assay' and 'A. cepa'. Only 18 papers met the inclusion criteria. None of these were published in the first eight years (2000-2007), highlighting the increasing interest in using the comet assay on A. cepa to analyse environmental samples over the last decade. The majority of the selected studies (15/18, 83%) were performed on samples belonging to the water compartment on onion bulbs. Half of the selected studies (9/18) were conducted to demonstrate the DNA damaging effect of the sample, while the other half of the studies not only recognized the presence of genotoxic agents but also addressed possible remediation measures. Detailed analysis of the experimental procedures revealed heterogeneity in many key steps, such as exposure time, test controls, nuclei isolation solutions, duration of electrophoresis, and number of nuclei scored. This literature review has shown that the comet assay on A. cepa, although recognized as an appropriate tool, is underutilized in environmental toxicology. Greater standardization could lead to its more widespread use, providing valuable information on the genotoxicity of environmental samples and the ability of different processes to mitigate their negative effects on plants. (© The Author(s) 2024. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
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