Protocol for producing a rat model of non-obese prediabetes using a mild hypercaloric diet approach.
| Autor: | Dwaib HS; Department of Clinical Nutrition and Dietetics, Faculty of Allied Medical Sciences, Palestine Ahliya University, Bethlehem 1041, Palestine. Electronic address: haneen.dwaib@paluniv.edu.ps., El-Yazbi AF; Research & Innovation Hub, Alamein International University, Alamein 51718, Egypt; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt. Electronic address: ayazbi@aiu.edu.eg. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103276. Date of Electronic Publication: 2024 Aug 21. |
| DOI: | 10.1016/j.xpro.2024.103276 |
| Abstrakt: | Metabolic disease complications pose a significant health risk due to their early development, making their diagnosis and radical therapy a considerable challenge. Here, we present a protocol for producing a rat model of non-obese prediabetes characterized by hyperinsulinemia, normoglycemia, and normal body weight. We describe steps for inducing the model in Sprague-Dawley (SD) male and ovariectomized female rats by free feeding on a mild hypercaloric diet. This protocol offers a potential model of metabolically unhealthy lean individuals. For complete details on the use and execution of this protocol, please refer to Elkhatib et al. 1 and Dwaib et al. 2 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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