| Autor: |
Shih CP; Institute of Physics, Academia Sinica, Taipei 11529, Taiwan.; Department of Chemistry, National Taiwan University, Taipei 106319, Taiwan.; Nano Science and Technology Program, Taiwan International Graduate Program, Academia Sinica and National Taiwan University, Taipei 11529, Taiwan., Tang WC; Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan., Chen P; Institute of Physics, Academia Sinica, Taipei 11529, Taiwan.; Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan., Chen BC; Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan. |
| Jazyk: |
angličtina |
| Zdroj: |
The journal of physical chemistry. B [J Phys Chem B] 2024 Sep 05; Vol. 128 (35), pp. 8273-8289. Date of Electronic Publication: 2024 Aug 23. |
| DOI: |
10.1021/acs.jpcb.4c01721 |
| Abstrakt: |
This Review explores the evolution, improvements, and recent applications of Light Sheet Fluorescence Microscopy (LSFM) in biological research using a high numerical aperture detection objective (lens) for imaging subcellular structures. The Review begins with an overview of the development of LSFM, tracing its evolution from its inception to its current state and emphasizing key milestones and technological advancements over the years. Subsequently, we will discuss various improvements of LSFM techniques, covering advancements in hardware such as illumination strategies, optical designs, and sample preparation methods that have enhanced imaging capabilities and resolution. The advancements in data acquisition and processing are also included, which provides a brief overview of the recent development of artificial intelligence. Fluorescence probes that were commonly used in LSFM will be highlighted, together with some insights regarding the selection of potential probe candidates for future LSFM development. Furthermore, we also discuss recent advances in the application of LSFM with a focus on high numerical aperture detection objectives for various biological studies. For sample preparation techniques, there are discussions regarding fluorescence probe selection, tissue clearing protocols, and some insights into expansion microscopy. Integrated setups such as adaptive optics, single objective modification, and microfluidics will also be some of the key discussion points in this Review. We hope that this comprehensive Review will provide a holistic perspective on the historical development, technical enhancements, and cutting-edge applications of LSFM, showcasing its pivotal role and future potential in advancing biological research. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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