Genomics of Terpene Biosynthesis in Dictyoceratid Sponges (Porifera) - What Do We (Not) Know?

Autor: Galitz A; Department of Earth- and Environmental Sciences, Palaeontology & Geobiology, Ludwig-Maximilians-Universität München, 80333, Munich, Germany., Vargas S; Department of Earth- and Environmental Sciences, Palaeontology & Geobiology, Ludwig-Maximilians-Universität München, 80333, Munich, Germany., Thomas OP; School of Biological and Chemical Sciences and Ryan Institute, University of Galway, H91TK33, Galway, Ireland., Reddy MM; School of Biological and Chemical Sciences and Ryan Institute, University of Galway, H91TK33, Galway, Ireland.; Department of Biological Sciences, University of Cape Town, Private Bag X3, 7701, Rondebosch, South Africa., Wörheide G; Department of Earth- and Environmental Sciences, Palaeontology & Geobiology, Ludwig-Maximilians-Universität München, 80333, Munich, Germany.; GeoBio-Center, Ludwig-Maximilians-Universität München, 80333, Munich, Germany.; SNSB-Bavarian State Collection of Palaeontology and Geology, 80333, Munich, Germany., Erpenbeck D; Department of Earth- and Environmental Sciences, Palaeontology & Geobiology, Ludwig-Maximilians-Universität München, 80333, Munich, Germany.; GeoBio-Center, Ludwig-Maximilians-Universität München, 80333, Munich, Germany.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2024 Sep; Vol. 21 (9), pp. e202400549. Date of Electronic Publication: 2024 Aug 23.
DOI: 10.1002/cbdv.202400549
Abstrakt: Sponges are recognized as promising sources for novel bioactive metabolites. Among them are terpenoid metabolites that constitute key biochemical defense mechanisms in several sponge taxa. Despite their significance, the genetic basis for terpenoid biosynthesis in sponges remains poorly understood. Dictyoceratida comprise demosponges well-known for their bioactive terpenoids. In this study, we explored the currently available genomic data for insights into the metabolic pathways of dictyoceratid terpenoids. We first identified prenyltransferase (PT) and terpene cyclase (TC) enzymes essential for the terpenoid biosynthetic processes in the terrestrial realm by analyzing available transcriptomic and genomic data of Dictyoceratida sponges and 10 other sponge species. All Dictyoceratida sponges displayed various PTs involved in either sesqui- or diterpene, steroid and carotenoid production. Additionally, it was possible to identify a potential candidate for a dictyoceratid sesterterpene PT. However, analogs of common terrestrial TCs were absent, suggesting the existence of a distinct or convergently evolved sponge-specific TC. Our study aims to contribute to the foundational understanding of terpene biosynthesis in sponges, unveiling the currently evident genetic components for terpenoid production in species not previously studied. Simultaneously, it aims to identify the known and unknown factors, as a starting point for biochemical and genetic investigations in sponge terpenoid production.
(© 2024 The Author(s). Chemistry & Biodiversity published by Wiley-VHCA AG.)
Databáze: MEDLINE
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