Successful transposition of sperm freezing, in vitro fertilization and artificial insemination methodologies in the Tadjik markhor species ( Capra falconeri heptneri ).

Autor: Locatelli Y; Muséum National d'Histoire Naturelle, Laboratoire de la Réserve Zoologique de la Haute Touche, Obterre, France.; Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Centre National de la Recherche Scientifique, Université de Tours, Nouzilly, France., Vion C; Muséum National d'Histoire Naturelle, Laboratoire de la Réserve Zoologique de la Haute Touche, Obterre, France., Duffard N; Muséum National d'Histoire Naturelle, Laboratoire de la Réserve Zoologique de la Haute Touche, Obterre, France., Bon N; Muséum National d'Histoire Naturelle, Laboratoire de la Réserve Zoologique de la Haute Touche, Obterre, France., Bernard J; Muséum National d'Histoire Naturelle, Laboratoire de la Réserve Zoologique de la Haute Touche, Obterre, France., Binaud C; Muséum National d'Histoire Naturelle, Laboratoire de la Réserve Zoologique de la Haute Touche, Obterre, France., Trontti N; Helsinki Zoo Korkeasaari, Helsinki, Finlande., Baril G; Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Centre National de la Recherche Scientifique, Université de Tours, Nouzilly, France., Mermillod P; Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Centre National de la Recherche Scientifique, Université de Tours, Nouzilly, France.
Jazyk: angličtina
Zdroj: Animal reproduction [Anim Reprod] 2024 Aug 12; Vol. 21 (3), pp. e20240071. Date of Electronic Publication: 2024 Aug 12 (Print Publication: 2024).
DOI: 10.1590/1984-3143-AR2024-0071
Abstrakt: The objective of the present study was to transpose sperm freezing methodology from domestic goat to the Tadjik markhor ( Capra falconeri heptneri ) and to address the feasibility to develop IVP and artificial insemination using such frozen semen. Semen of different adult markhor males were successfully recovered by electro-ejaculation and were then frozen using caprine methodology. Frozen semen showed good survival rates at thawing and good fertility rates were assessed in heterologous in vitro fertilization system with goat oocytes. LOPU/IVF was applied for Tadjik markhor females allowing the first successful blastocyst production in vitro . In an applied program, we also transposed successfully intrauterine AI method with frozen/thawed semen to the Tadjik markhor.
Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare.
(Copyright © The Author(s).)
Databáze: MEDLINE