Characterization of cellulases from softening fruit for enzymatic depolymerization of cellulose.

Autor: Edema H; The Arctic Centre for Sustainable Energy, UiT The Arctic University of Norway, Tromsø 9037, Norway; Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø 9037, Norway. Electronic address: hilary.edema@uit.no., Ashraf MF; The Arctic Centre for Sustainable Energy, UiT The Arctic University of Norway, Tromsø 9037, Norway; Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø 9037, Norway. Electronic address: muhammad.f.ashraf@uit.no., Samkumar A; Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø 9037, Norway; Department of Plant Science, Norwegian University of Life Sciences, Ås 1430, Norway. Electronic address: amos.s.premkumar@nmbu.no., Jaakola L; Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø 9037, Norway; Division of Food Production and Society, Norwegian Institute of Bioeconomy Research (NIBIO), Ås 1431, Norway. Electronic address: laura.jaakola@uit.no., Karppinen K; The Arctic Centre for Sustainable Energy, UiT The Arctic University of Norway, Tromsø 9037, Norway; Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø 9037, Norway. Electronic address: katja.karppinen@uit.no.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2024 Nov 01; Vol. 343, pp. 122493. Date of Electronic Publication: 2024 Jul 14.
DOI: 10.1016/j.carbpol.2024.122493
Abstrakt: Cellulose is a major renewable resource for a wide variety of sustainable industrial products. However, for its utilization, finding new efficient enzymes for plant cell wall depolymerization is crucial. In addition to microbial sources, cellulases also exist in plants, however, are less studied. Fleshy fruit ripening includes enzymatic cell wall hydrolysis, leading to tissue softening. Therefore, bilberry (Vaccinium myrtillus L.), which produces small fruits that undergo extensive and rapid softening, was selected to explore cellulases of plant origin. We identified 20 glycoside hydrolase family 9 (GH9) cellulases from a recently sequenced bilberry genome, including four of which showed fruit ripening-specific expression and could be associated with fruit softening based on phylogenetic, transcriptomic and gene expression analyses. These four cellulases were secreted enzymes: two B-types and two C-types with a carbohydrate binding module 49. For functional characterization, these four cellulases were expressed in Pichia pastoris. All recombinant enzymes demonstrated glucanase activity toward cellulose and hemicellulose substrates. Particularly, VmGH9C1 demonstrated high activity and ability to degrade cellulose, xyloglucan, and glucomannan. In addition, all the enzymes retained activity under wide pH (6-10) and temperature ranges (optimum 70 °C), revealing the potential applications of plant GH9 cellulases in the industrial bioprocessing of lignocellulose.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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