The clinical importance of IFN-γ and human epididymis protein 4 in Egyptian patients with epithelial ovarian cancer combined with HPV infection.

Autor: Mohamed NE; Zoology Department, Faculty of Science, Cairo University, Cairo, Egypt., Abdel Fattah NF; Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt., Seadawy MG; Biodefense Center for Infectious and Emerging Diseases, Ministry of Defense, Cairo, Egypt., Lymona AM; Surgical Oncology Department, National Cancer Institute, Cairo University, Cairo, Egypt., Nasr SS; Cancer Epidemiology and Biostatistics Department, National Cancer Institute, Cairo University, Cairo, Egypt., El Leithy AA; College of Biotechnology, Misr University for Science and Technology, Giza, Egypt., Abdelwahed FM; Medical Biochemistry and Molecular Biology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt., Nassar A; Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt. Electronic address: Auhood.nassar@nci.cu.edu.eg.
Jazyk: angličtina
Zdroj: Human immunology [Hum Immunol] 2024 Sep; Vol. 85 (5), pp. 111089. Date of Electronic Publication: 2024 Aug 21.
DOI: 10.1016/j.humimm.2024.111089
Abstrakt: Background: High-grade Epithelial Ovarian Cancer (HGEOC) is an aggressive disease that usually presents at an advanced stage. Thus, detecting the circulating cytokines (IFNγ and TNF-α) may serve as a biomarker to identify malignancy and manage therapeutic decisions.
Objectives: Assessing the clinical importance of inflammatory mediators and tumor markers in EOC Egyptian patients compared with benign cases. Moreover, identifying the distinct inflammatory mediators in EOC patients combined with HPV infection.
Methods: This study was conducted on 61 Egyptian patients, divided into 25 patients with HGEOC, 22 patients with LGEOC, and 14 benign ovarian tumor cases. Measurements of serum HE4, CA125, CEA, and CA19-9 were determined by Roche Elecsys immunoassays. Serum levels of TNF-α and IFN-γ were measured using quantitative sandwich ELISA. Quantitative genotyping of HPV DNA types 16, 18, and 45 was assessed for the HPV DNA-positive samples.
Results: HPV DNA was detected in 25.53 % of malignant cases, HPV 16 was detected in 50 % of HPV-positive cases, and only 1 case of HPV 18 was detected out of 12 positive cases. The Human Epididymis protein 4 (HE4) was statistically different between patients with EOC and benign cases (p-value = 0.007) and between HPV DNA positive and HPV DNA negative cases (p-value = 0.008). The serum levels of IFN- γ were statistically different between HGEOC and LGEOC (p-value < 0.001), while the serum levels of TNF-α didn't differ statistically between the two groups.
Conclusion: IFN-γ could be used as a biomarker to discriminate HGEOC and LGEOC. Initial evidence for the possible association between HE4 and the progression of HPV-associated EOC was speculated.
(Copyright © 2024 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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