IgT-mediated mucosal immunity and microbiota dynamics in snakehead (Channa argus) post Aeromonas veronii TH0426 and Aeromonas hydrophila TPS infection: implications for aquaculture disease management.

Autor: Liu YM; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, 132101, Jilin, China., Li XT; College of Animal Science and Technology, College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, Jilin, China., Zhang CY; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, 132101, Jilin, China., Li CH; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, 132101, Jilin, China., Wang HY; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, 132101, Jilin, China., Zhang DX; College of Animal Science and Technology, College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, Jilin, China., Zhang L; College of Animal Science and Technology, College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, Jilin, China., Sun WW; College of Animal Science and Technology, College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, Jilin, China., Tao LT; College of Animal Science and Technology, College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, Jilin, China. zephyr1994@foxmail.com., Shan XF; College of Animal Science and Technology, College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, Jilin, China.
Jazyk: angličtina
Zdroj: International microbiology : the official journal of the Spanish Society for Microbiology [Int Microbiol] 2024 Aug 22. Date of Electronic Publication: 2024 Aug 22.
DOI: 10.1007/s10123-024-00581-z
Abstrakt: The aquaculture sector, vital to global food security, grapples with bacterial pathogens compromising fish health and industry sustainability. This investigation probes mucosal immune responses and gut microbiota dynamics in snakehead (Channa argus) post-Aeromonas infection, a prevalent aquaculture challenge. Employing infection models, we delineated the integral role of immunoglobulin T (IgT) in mucosal immunity and its interaction with gut microbiota. Fish from a local farm, maintained under controlled conditions, were infected with Aeromonas veronii TH0426 and Aeromonas hydrophila TPS. Post-infection, daily monitoring and sample collection at specified intervals were conducted for comprehensive analysis. Histopathology, quantitative PCR, immunofluorescence, and microbiota profiling revealed significant immune and microbial changes, particularly at day 7. Intestinal IgT, IgM, and pIgR gene expression surged, indicative of a robust response. Immunofluorescence microscopy confirmed increased IgT + and pIgR + cell infiltration in the epithelium. Post-infection dysbiosis, with altered bacterial composition, was partially offset by elevated IgT levels. These insights underscore IgT's crucial function in mucosal defense and suggest potential for probiotic and vaccine strategies to enhance aquaculture disease resilience.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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