Real-time monitoring of voltage-responsive biomolecular binding onto electro-switchable surfaces.

Autor: Pringle NE; Department of Chemistry and Biochemistry, Brigham Young University, C100 BNSN, Provo, UT, 84602, USA., Mendes PM; School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK., Paxton WF; Department of Chemistry and Biochemistry, Brigham Young University, C100 BNSN, Provo, UT, 84602, USA. paxton@chem.byu.edu.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2024 Nov; Vol. 416 (26), pp. 5743-5751. Date of Electronic Publication: 2024 Aug 22.
DOI: 10.1007/s00216-024-05493-5
Abstrakt: Voltage-responsive biosensors capable of monitoring real-time adsorption behavior of biological analytes onto electroactive surfaces offer attractive strategies for disease detection, separations, and other adsorption-dependent analytical techniques. Adsorption of biological analytes onto electrically switchable surfaces can be modelled using neutravidin and biotin. Here, we report self-assembled monolayers formed from voltage-switchable biotinylated molecules on gold surfaces with tunable sensitivity to neutravidin in response to applied voltages. By using electrochemical quartz crystal microbalance (EQCM), we demonstrated real-time switchable behavior of these bio-surfaces and investigate the range of sensitivity by varying the potential of the same surfaces from -400 mV to open circuit potential (+155 mV) to +300 mV. We compared the tunability of the mixed surfaces to bare Au surfaces, voltage inert surfaces, and switchable biotinylated surfaces. Our results indicate that quartz crystal microbalance allows real-time changes in analyte binding behavior, which enabled observing the evolution of neutravidin sensitivity as the applied voltage was shifted. EQCM could in principle be used in kinetic studies or to optimize voltage-switchable surfaces in adsorption-based diagnostics.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)
Databáze: MEDLINE
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