Stability of adrenocorticotropic hormone in whole blood samples: effects of storage conditions.

Autor: Fraissinet F; General Biochemistry Department, Institute for Clinical Biology, Rouen University Hospital, Rouen, France.; UNIROUEN, Normandie University, INSERM, U1239, NorDiC, Rouen, France., Girot H; General Biochemistry Department, Institute for Clinical Biology, Rouen University Hospital, Rouen, France., Gillibert A; Biostatistics Department, Rouen University Hospital, Rouen, France., Melin A; General Biochemistry Department, Institute for Clinical Biology, Rouen University Hospital, Rouen, France., Fettig J; General Biochemistry Department, Institute for Clinical Biology, Rouen University Hospital, Rouen, France., Brunel V; General Biochemistry Department, Institute for Clinical Biology, Rouen University Hospital, Rouen, France.
Jazyk: angličtina
Zdroj: Biochemia medica [Biochem Med (Zagreb)] 2024 Oct 15; Vol. 34 (3), pp. 030702. Date of Electronic Publication: 2024 Aug 05.
DOI: 10.11613/BM.2024.030702
Abstrakt: Introduction: Adrenocorticotropic hormone (ACTH) is a peptide secreted by pituitary gland that plays an important role in regulating cortisol secretion. Its determination is difficult because of instability in whole blood. Several factors that influence ACTH stability in blood before analysis have been identified: temperature, hemolysis, time to centrifugation and presence of protease inhibitors. Published results on ACTH whole blood stability seem contradictory.
Materials and Methods: We performed a stability study in 10 healthy volunteers. Three different conditions were tested: ethylenediaminetetraacetic acid (EDTA) at 4 °C, EDTA + aprotinin at 4 °C, EDTA + aprotinin at room temperature. Stability was evaluated for 8 hours. Adrenocorticotropic hormone measurements and hemolysis index were performed respectively on Cobas e602 and c701 (Roche Diagnostics, Mannheim, Germany). We compared percentage deviations with total change limit using a threshold of 7.5%.
Results: We showed that ACTH is stable 8 hours with EDTA at 4 °C, 4 hours with EDTA + aprotinin at 4 °C and 2 hours with EDTA + aprotinin at 22 °C.
Conclusions: Aprotinin does not appear to give ACTH greater stability but can be used without exceeding 4 hours at 4 °C. Refrigerated pouch transport also seems to be more appropriate for ACTH in whole blood.
Competing Interests: Potential conflict of interest None declared.
(Croatian Society of Medical Biochemistry and Laboratory Medicine.)
Databáze: MEDLINE
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