Extracellular vesicles may provide an alternative detoxification pathway during skeletal muscle myoblast ageing.

Autor: Fernández-Rhodes M; School of Sport Exercise and Health Sciences, Loughborough University Loughborough UK., Buchan E; School of Chemical Engineering, Advanced Nanomaterials Structures and Applications Laboratories, College of Engineering and Physical Sciences University of Birmingham Birmingham UK., Gagnon SD; School of Sport Exercise and Health Sciences, Loughborough University Loughborough UK., Qian J; School of Sport Exercise and Health Sciences, Loughborough University Loughborough UK., Gethings L; Waters Corporation Wilmslow UK.; School of Biological Sciences University of Manchester Manchester UK.; Medical School University of Surrey Surrey UK., Lees R; NanoFCM Co., LTD Nottingham Nottinghamshire UK., Peacock B; School of Biological Sciences University of Manchester Manchester UK., Capel AJ; School of Sport Exercise and Health Sciences, Loughborough University Loughborough UK., Martin NRW; School of Sport Exercise and Health Sciences, Loughborough University Loughborough UK., Oppenheimer PG; School of Chemical Engineering, Advanced Nanomaterials Structures and Applications Laboratories, College of Engineering and Physical Sciences University of Birmingham Birmingham UK., Lewis MP; School of Sport Exercise and Health Sciences, Loughborough University Loughborough UK., Davies OG; School of Sport Exercise and Health Sciences, Loughborough University Loughborough UK.
Jazyk: angličtina
Zdroj: Journal of extracellular biology [J Extracell Biol] 2024 Aug 21; Vol. 3 (8), pp. e171. Date of Electronic Publication: 2024 Aug 21 (Print Publication: 2024).
DOI: 10.1002/jex2.171
Abstrakt: Skeletal muscle (SM) acts as a secretory organ, capable of releasing myokines and extracellular vesicles (SM-EVs) that impact myogenesis and homeostasis. While age-related changes have been previously reported in murine SM-EVs, no study has comprehensively profiled SM-EV in human models. To this end, we provide the first comprehensive comparison of SM-EVs from young and old human primary skeletal muscle cells (HPMCs) to map changes associated with SM ageing. HPMCs, isolated from young (24 ± 1.7 years old) and older (69 ± 2.6 years old) participants, were immunomagnetically sorted based on the presence of the myogenic marker CD56 (N-CAM) and cultured as pure (100% CD56 + ) or mixed populations (MP: 90% CD56 + ). SM-EVs were isolated using an optimised protocol combining ultrafiltration and size exclusion chromatography (UF + SEC) and their biological content was extensively characterised using Raman spectroscopy (RS) and liquid chromatography mass spectrometry (LC-MS). Minimal variations in basic EV parameters (particle number, size, protein markers) were observed between young and old populations. However, biochemical fingerprinting by RS highlighted increased protein (amide I), lipid (phospholipids and phosphatidylcholine) and hypoxanthine signatures for older SM-EVs. Through LC-MS, we identified 84 shared proteins with functions principally related to cell homeostasis, muscle maintenance and transcriptional regulation. Significantly, SM-EVs from older participants were comparatively enriched in proteins involved in oxidative stress and DNA/RNA mutagenesis, such as E3 ubiquitin-protein ligase TTC3 (TTC3), little elongation complex subunit 1 (ICE1) and Acetyl-CoA carboxylase 1 (ACACA). These data suggest SM-EVs could provide an alternative pathway for homeostasis and detoxification during SM ageing.
Competing Interests: The authors declare no conflicts of interest.
(© 2024 The Author(s). Journal of Extracellular Biology published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.)
Databáze: MEDLINE
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