Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria.

Autor: Simpson A; Sport and Physical Activity Research Institute, University of the West of Scotland, Blantyre, UK., Johnston W; School of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow, UK., Carda-Diéguez M; Department of Health and Genomics, Centre for Advanced Research in Public Health, FISABIO Foundation, Valencia, Spain., Mira A; Department of Health and Genomics, Centre for Advanced Research in Public Health, FISABIO Foundation, Valencia, Spain., Easton C; Sport and Physical Activity Research Institute, University of the West of Scotland, Blantyre, UK., Henriquez FL; Sport and Physical Activity Research Institute, University of the West of Scotland, Blantyre, UK., Culshaw S; Oral Sciences, University of Glasgow Dental School, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK., Rosier BT; Department of Health and Genomics, Centre for Advanced Research in Public Health, FISABIO Foundation, Valencia, Spain., Burleigh M; Sport and Physical Activity Research Institute, University of the West of Scotland, Blantyre, UK.
Jazyk: angličtina
Zdroj: Molecular oral microbiology [Mol Oral Microbiol] 2024 Dec; Vol. 39 (6), pp. 491-506. Date of Electronic Publication: 2024 Aug 22.
DOI: 10.1111/omi.12479
Abstrakt: Background: The oral microbiome-dependent nitrate (NO 3 - )-nitrite (NO 2 - )-nitric oxide (NO) pathway may help regulate blood pressure. NO 2 - -producing bacteria in subgingival plaque are reduced in relative abundance in patients with untreated periodontitis compared with periodontally healthy patients. In periodontitis patients, the NO 2 - -producing bacteria increase several months after periodontal treatment. The early effects of periodontal treatment on NO 2 - -producing bacteria and the NO 3 - -NO 2 - -NO pathway remain unknown. The aim of this study was to determine how periodontal treatment affects the oral NO 2 - -producing microbiome and salivary NO 3 - and NO 2 - levels over time.
Methods: The subgingival microbiota of 38 periodontitis patients was analysed before (baseline [BL]) and 1, 7 and 90 days after periodontal treatment. Changes in NO 2 - -producing bacteria and periodontitis-associated bacteria were determined by 16s rRNA Illumina sequencing. Saliva samples were collected at all-time points to determine NO 3 - and NO 2 - levels using gas-phase chemiluminescence.
Results: A significant increase was observed in the relative abundance of NO 2 - -producing species between BL and all subsequent timepoints (all p < 0.001). Periodontitis-associated species decreased at all timepoints, relative to BL (all p < 0.02). NO 2 - -producing species negatively correlated with periodontitis-associated species at all timepoints, with this relationship strongest 90 days post-treatment (ρ = -0.792, p < 0.001). Despite these findings, no significant changes were found in salivary NO 3 - and NO 2 - over time (all p > 0.05).
Conclusions: Periodontal treatment induced an immediate increase in the relative abundance of health-associated NO 2 - -producing bacteria. This increase persisted throughout periodontal healing. Future studies should test the effect of periodontal treatment combined with NO 3 - intake on periodontal and cardiovascular health.
(© 2024 The Author(s). Molecular Oral Microbiology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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