Effect of seminal plasma cholesterol and triacylglycerides concentrations and sperm morphology on semen freezability in domestic cats (Felis silvestris catus).

Autor: García MF; National Scientific and Technical Research Council -CONICET, CABA, Argentina; Instituto de Investigaciones en Reproducción Animal, Faculty of Veterinary Sciences, La Plata National University, Argentina., Favre RN; National Scientific and Technical Research Council -CONICET, CABA, Argentina; Instituto de Investigaciones en Reproducción Animal, Faculty of Veterinary Sciences, La Plata National University, Argentina., Rearte R; National Scientific and Technical Research Council -CONICET, CABA, Argentina; Instituto de Investigaciones en Reproducción Animal, Faculty of Veterinary Sciences, La Plata National University, Argentina., Stornelli MC; Instituto de Investigaciones en Reproducción Animal, Faculty of Veterinary Sciences, La Plata National University, Argentina., Jurado S; Instituto de Investigaciones en Reproducción Animal, Faculty of Veterinary Sciences, La Plata National University, Argentina., de la Sota L; National Scientific and Technical Research Council -CONICET, CABA, Argentina; Instituto de Investigaciones en Reproducción Animal, Faculty of Veterinary Sciences, La Plata National University, Argentina., Stornelli MA; Instituto de Investigaciones en Reproducción Animal, Faculty of Veterinary Sciences, La Plata National University, Argentina. Electronic address: astornel@fcv.unlp.edu.ar.
Jazyk: angličtina
Zdroj: Topics in companion animal medicine [Top Companion Anim Med] 2024 Sep-Oct; Vol. 62, pp. 100907. Date of Electronic Publication: 2024 Aug 20.
DOI: 10.1016/j.tcam.2024.100907
Abstrakt: There is scarce information about the effect of sperm morphology and seminal plasma composition on cat semen freezability. Thus, this study aims to assess the effect of cat sperm morphology and seminal plasma cholesterol (CHOL) and triacylglyceride (TAG) concentrations on sperm post-thaw survival. Ejaculates (n = 49) were evaluated, and seminal plasma was separated and frozen until CHOL and TAG concentrations were measured. The sperm pellet was diluted in a tris-based egg yolk extender, frozen (n = 38), or processed for sperm ultrastructure study (n = 11). Abnormalities recorded were abnormal head shape and size, detached heads, knobbed or ruffled acrosomes, eccentric mid-piece insertion, proximal and distal cytoplasmic droplets, folded and coiled tails, and Dag defect. Ultramicroscopic evaluation detected several sperm abnormalities in fresh semen and some sperm damage in frozen semen. Seminal plasma lipids components were positively correlated with post-thaw motility and acrosome integrity. Higher freezability indices for motility and acrosome integrity were observed in frozen-thawed semen with high seminal plasma CHOL and TAG concentrations. No freezability differences were observed between teratozoospermic and normozoospermic ejaculates. Our results showed that even when seminal plasma was removed before cryopreservation, sperm survival after thawing was significantly higher in samples with high seminal plasma CHOL and TAG concentrations, indicating a rapid adherence to these compounds to the sperm plasma membrane, protecting sperm cells from temperature changes. Nevertheless, there were no differences in sperm freezability by sperm morphology.
Competing Interests: Declaration of competing interest None.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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