Methods for monitoring protein-membrane binding. Comparison based on the interactions between amyloidogenic protein human cystatin C and phospholipid liposomes.

Autor: Orlikowska M; Department of Biomedical Chemistry, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland. Electronic address: marta.orlikowska@ug.edu.pl., Wyciszkiewicz A; Sartorius BioAnalytics, Wrzesinska 70/A, 62-025 Kostrzyn Wielkopolski, Poland. Electronic address: aleksandra.wyciszkiewicz@sartorius.com., Węgrzyn K; Laboratory of Molecular Biology, Intercollegiate Faculty of Biotechnology UG&MUG, University of Gdansk, Abrahama 58, 80-307 Gdansk, Poland. Electronic address: katarzyna.wegrzyn@biotech.ug.edu.pl., Mehringer J; Dynamic Biosensors GmbH, Perchtinger Str. 8-10, 81379 Munich, Germany. Electronic address: johannes.mehringer@dynamic-biosensors.com., de Souza Paiva D; Dynamic Biosensors GmbH, Perchtinger Str. 8-10, 81379 Munich, Germany. Electronic address: daisy.paiva@dynamic-biosensors.com., Jurczak P; Laboratory of Mass Spectrometry, Intercollegiate Faculty of Biotechnology UG&MUG, University of Gdansk, Abrahama 58, 80-307 Gdansk, Poland; Biomacromolecule Research Team, RIKEN Center for Sustainable Resource Science, Wako-shi, Saitama 351-0198, Japan. Electronic address: przemyslaw.jurczak@ug.edu.pl.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Oct; Vol. 278 (Pt 3), pp. 134889. Date of Electronic Publication: 2024 Aug 19.
DOI: 10.1016/j.ijbiomac.2024.134889
Abstrakt: A cell membrane is an essential cellular component providing protection against the outer environment. It is also a host for proteins and carbohydrates responsible for, e.g. transporter, receptor, or enzymatic functions. In parallel, the membrane may also be implicated in pathological processes leading, e.g. to the oligomerization of amyloid-forming proteins, a hallmark of i.a. Alzheimer's disease. The increasing need for detailed information on mechanisms driving the amyloid formation and the potential role of cell membranes in the process proves the research on protein-membrane interactions biologically relevant. Considering the potential and limitations of the relatively well established and newly developed methods, this study focused on selecting methods that allow a broad and comprehensive description of interactions between amyloidogenic protein human cystatin C and lipid bilayers. In the first step, dot-blot and ELISA tests were selected as techniques allowing fast screening for protein-ligand interactions. Next, surface plasmon resonance, spectral shift, biolayer interferometry, and switchSENSE® technology were used to determine kinetic parameters and binding constants for interactions between human cystatin C and the selected lipid bilayers. Based on the obtained results we have proposed the most promising candidates for monitoring of interactions and determining affinity between amyloidogenic proteins and membrane mimetics.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Przemyslaw Jurczak reports financial support was provided by National Science Centre Poland. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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