Monitoring of pathogenic Leptospira infection in wolves (Canis lupus) from Spain and Italy.

Autor: Gonzálvez M; Departamento de Sanidad Animal, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Córdoba 14014, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad de Murcia, Murcia 30100, Spain., Cano-Terriza D; Departamento de Sanidad Animal, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Córdoba 14014, Spain; CIBERINFEC, ISCIII CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid 28029, Spain. Electronic address: v82cated@uco.es., Fayos M; Centro de Recuperación de Fauna Silvestre de Cantabria, Tragsatec, Dirección General de Montes y Biodiversidad Cantabria, Gobierno de Cantabria, Santander, Spain., Moroni B; Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, Torino 10154, Italy., Martínez R; Departamento de Sanidad Animal, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Córdoba 14014, Spain., Robetto S; Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Centro di Referenza Nazionale Malattie Animali Selvatici (CERMAS), Località Amerique 7G, Quart 11020, Italy., Oleaga Á; SERPA, Sociedad de Servicios del Principado de Asturias S.A., La Laboral, Gijón 33203, Spain., Remesar S; Grupo INVESAGA, Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo 27002, Spain; IBADER, Instituto de Biodiversidade Agraria e Desenvolvemento Rural, Lugo, Spain., Orusa R; Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Centro di Referenza Nazionale Malattie Animali Selvatici (CERMAS), Località Amerique 7G, Quart 11020, Italy., Muñoz-Hernández C; Departamento de Sanidad Animal, Facultad de Veterinaria, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad de Murcia, Murcia 30100, Spain; Grupo SaBio, Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC, UCLM, JCCM), Ciudad Real 13005, Spain., Velarde R; Wildlife Ecology & Health group (WEH) and Servei d'Ecopatologia de Fauna Salvatge (SEFaS), Departament de Medicina i Cirugia Animals, Universitat Autònoma de Barcelona, Bellaterra, Spain., García-Bocanegra I; Departamento de Sanidad Animal, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Córdoba 14014, Spain; CIBERINFEC, ISCIII CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid 28029, Spain.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2024 Aug 15; Vol. 298, pp. 110222. Date of Electronic Publication: 2024 Aug 15.
DOI: 10.1016/j.vetmic.2024.110222
Abstrakt: Leptospirosis is a bacterial disease of worldwide distribution with relevant implications for animal and human health. Different large wild carnivore species can act as reservoirs of this zoonotic pathogen. This study aimed to evaluate the circulation of Leptospira spp. in free-ranging wolves (Canis lupus) from southern Europe. A total of 281 kidney samples of wolves from Spain and Italy were collected between 2017 and 2023. The presence of Leptospira DNA was analysed by real-time PCR and phylogenetic analyses were carried out using a Bayesian approach. The overall prevalence was 3.2 % (9/281; 95 %CI: 1.1-5.3). Leptospira DNA was detected in nine of the 180 wolves from Spain (5.0 %; 95 %CI: 1.8-8.2), but not in the Italian wolf population (0 %; 0/101). Molecular analyses revealed high homology between the sequences obtained in the present study and isolates of Leptospira interrogans and Leptospira borgpetersenii from different rodent and domestic ungulate species. Our results provide evidence of a low and spatially heterogeneous circulation of this pathogen in wolf populations of southern Europe. The detection of zoonotic Leptospira species in this survey supports the need to consider wolf populations in monitoring programs for leptospirosis with a One Health approach.
Competing Interests: Declaration of Competing Interest None of the authors of this study has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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