A highly sensitive electrochemical immunosensor based on rGO-PEI-Ag nanocomposites for the detection of tilmicosin.

Autor: Chen Y; School of Life Sciences, Zhengzhou University, No. 100, Science Avenue, 450001, Zhengzhou City, Henan Province, People's Republic of China; Longhu Laboratory, No. 218, Ping AN Avenue, Zhengdong New District, 450046, Zhengzhou City, Henan Province, People's Republic of China., Zhang J; School of Life Sciences, Zhengzhou University, No. 100, Science Avenue, 450001, Zhengzhou City, Henan Province, People's Republic of China., Liu R; School of Life Sciences, Zhengzhou University, No. 100, Science Avenue, 450001, Zhengzhou City, Henan Province, People's Republic of China., Zhang Y; School of Life Sciences, Zhengzhou University, No. 100, Science Avenue, 450001, Zhengzhou City, Henan Province, People's Republic of China; Longhu Laboratory, No. 218, Ping AN Avenue, Zhengdong New District, 450046, Zhengzhou City, Henan Province, People's Republic of China., Zhou J; School of Life Sciences, Zhengzhou University, No. 100, Science Avenue, 450001, Zhengzhou City, Henan Province, People's Republic of China; Longhu Laboratory, No. 218, Ping AN Avenue, Zhengdong New District, 450046, Zhengzhou City, Henan Province, People's Republic of China., Liu H; School of Life Sciences, Zhengzhou University, No. 100, Science Avenue, 450001, Zhengzhou City, Henan Province, People's Republic of China; Longhu Laboratory, No. 218, Ping AN Avenue, Zhengdong New District, 450046, Zhengzhou City, Henan Province, People's Republic of China., Liu Y; School of Life Sciences, Zhengzhou University, No. 100, Science Avenue, 450001, Zhengzhou City, Henan Province, People's Republic of China; Longhu Laboratory, No. 218, Ping AN Avenue, Zhengdong New District, 450046, Zhengzhou City, Henan Province, People's Republic of China., Yan K; School of Life Sciences, Zhengzhou University, No. 100, Science Avenue, 450001, Zhengzhou City, Henan Province, People's Republic of China., Qi Y; School of Life Sciences, Zhengzhou University, No. 100, Science Avenue, 450001, Zhengzhou City, Henan Province, People's Republic of China; Longhu Laboratory, No. 218, Ping AN Avenue, Zhengdong New District, 450046, Zhengzhou City, Henan Province, People's Republic of China., Liu E; School of Life Sciences, Zhengzhou University, No. 100, Science Avenue, 450001, Zhengzhou City, Henan Province, People's Republic of China; Longhu Laboratory, No. 218, Ping AN Avenue, Zhengdong New District, 450046, Zhengzhou City, Henan Province, People's Republic of China., Zhu X; School of Life Sciences, Zhengzhou University, No. 100, Science Avenue, 450001, Zhengzhou City, Henan Province, People's Republic of China; Longhu Laboratory, No. 218, Ping AN Avenue, Zhengdong New District, 450046, Zhengzhou City, Henan Province, People's Republic of China., Wang A; School of Life Sciences, Zhengzhou University, No. 100, Science Avenue, 450001, Zhengzhou City, Henan Province, People's Republic of China; Longhu Laboratory, No. 218, Ping AN Avenue, Zhengdong New District, 450046, Zhengzhou City, Henan Province, People's Republic of China. Electronic address: pingaw@126.com.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Dec 15; Vol. 461, pp. 140009. Date of Electronic Publication: 2024 Jun 07.
DOI: 10.1016/j.foodchem.2024.140009
Abstrakt: A label-free electrochemical immunosensor was developed to rapidly detect tilmicosin (TMC) residues in pork and milk. The immunosensor was constructed by immobilizing a high-affinity monoclonal antibody against TMC on an rGO-PEI-Ag nanocomposite-modified electrode. The rGO-PEI-Ag nanocomposites were prepared by mixing polyethyleneimine (PEI) modified reduced graphene oxide (rGO) with AgNO 3 solution. The prepared rGO-PEI-Ag nanocomposites showed good redox activity and conductivity, as characterized by ultraviolet-visible spectroscopy (UV-Vis), transmission electron microscopy (TEM), and X-ray diffraction (XRD). During the preparation process, staphylococcal protein A (SPA) was added to targetedly bind the Fc segment of the monoclonal antibody. The immunosensor showed a low detection limit (LOD) of 0.0013 ng/mL, a linear range of 0.01-100 ng/mL, and recoveries ranging from 92.77 to 100.02% in pork and 92.26-101.23% in milk. Furthermore, the immunosensor exhibited good stability, reproducibility, and specificity in detecting TMC in pork and milk real samples.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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