Antifungal, molecular docking and cytotoxic effect of the essential oil of Cymbopogon citratus (DC) Stapf. and Cymbopogon nardus (L.) Rendle against Candida albicans.

Autor:	Prado GM; Universidade Federal do Ceará, Av. Cmte. Maurocélio Rocha Pontes, 100, Jocely Dantas de Andrade Torres, 62042-250 Sobral, CE, Brazil., Prado JCS; Universidade Federal do Ceará, Av. Cmte. Maurocélio Rocha Pontes, 100, Jocely Dantas de Andrade Torres, 62042-250 Sobral, CE, Brazil., Aguiar FLL; Universidade Estadual Vale do Acaraú, Centro de Ciências Agrárias e Biológicas, Av. Padre Francisco Sadoc de Araujo, 850, Alto da Brasilia, 62010-295 Sobral, CE, Brazil., Barbosa FCB; Universidade Federal do Ceará, Av. Cmte. Maurocélio Rocha Pontes, 100, Jocely Dantas de Andrade Torres, 62042-250 Sobral, CE, Brazil., Vale JPCD; Universidade Estadual Vale do Acaraú, Centro de Ciências Exatas e Tecnologia, Av. Padre Francisco Sadoc de Araujo, 850, Alto da Brasilia, 62010-295 Sobral, CE, Brazil., Martins MR; Departmento de Ciências Médicas e da Saúde, Universidade de Evora, Colégio Luís António Verney, Rua Romão Ramalho, 59, 7000-671 Évora, Portugal.; HERCULES Laboratory, Instituto de Investigação e Formação Avançada (IIFA), Universidade de Evora, Palácio do Vimioso, Largo Marquês de Marialva 8, 7000-809, Evora, Portugal., Arantes SM; HERCULES Laboratory, Instituto de Investigação e Formação Avançada (IIFA), Universidade de Evora, Palácio do Vimioso, Largo Marquês de Marialva 8, 7000-809, Evora, Portugal., Sousa NV; Programa em Ciências Médicas, Universidade de Fortaleza, Centro de Ciências da Saúde, Av. da Universidade, 2853, Benfica, 60020-181 Fortaleza, CE, CEP, Brazil., Lima DM; Programa em Ciências Médicas, Universidade de Fortaleza, Centro de Ciências da Saúde, Av. da Universidade, 2853, Benfica, 60020-181 Fortaleza, CE, CEP, Brazil., Marinho ES; Programa de Pós-Graduação em Ciências Naturais, Universidade Estadual do Ceará/UECE, Av. Dr. Silas Munguba, 1700, 60714-903 Fortaleza, CE, Brazil., Marinho MM; Programa de Pós-Graduação em Ciências Naturais, Universidade Estadual do Ceará/UECE, Av. Dr. Silas Munguba, 1700, 60714-903 Fortaleza, CE, Brazil., Fontenelle ROS; Universidade Estadual Vale do Acaraú, Centro de Ciências Agrárias e Biológicas, Av. Padre Francisco Sadoc de Araujo, 850, Alto da Brasilia, 62010-295 Sobral, CE, Brazil.
Jazyk:	angličtina
Zdroj:	Anais da Academia Brasileira de Ciencias [An Acad Bras Cienc] 2024 Aug 16; Vol. 96 (3), pp. e20230309. Date of Electronic Publication: 2024 Aug 16 (Print Publication: 2024).
DOI:	10.1590/0001-3765202420230309
Abstrakt:	Brazil is renowned for its extensive plant biodiversity, with emphasis on Cymbopogon, C. citratus and C. nardus, with broad antimicrobial potential. Candidemias caused by Candida albicans are highly prevalent in immunosuppressed individuals and are associated with infections by biofilms on medical devices. The aim of this study was to evaluate the antimicrobial potential of essential oils C. citratus and C. nardus against C. albicans in planktonic and biofilm forms. Essential oils were obtained by hydrodistillation and chemical composition evaluated by GC-FID and GC-MS. The minimum inhibitory concentration was determined by the broth microdilution method and the synergy effect of essential oils and amphotericin B were evaluated by the checkerboard test. Biofilm activity was determined by the XTT assay. Cytotoxicity assays performed with VERO cells and molecular docking were performed to predict the effect of oil interaction on the SAP-5 enzyme site. The results showed activity of essential oils against planktonic cells and biofilm of C. albicans. Furthermore, the oils had a synergistic effect, and low cytotoxicity. Molecular docking showed interaction between Cadinene, Caryophyllen oxide, Germacrene D with SAP-5. The results indicate that Cymbopogon spp. studied are anti-Candida, with potential for further application in therapy against infections caused by C. albicans.
Databáze:	MEDLINE
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