Red propolis extract associated to platelet-rich plasma and stromal cells with focus in cell therapy and functional tissue regeneration.

Autor:	Garcia CSC; Universidade de Caxias do Sul, Institute of Biotechnology, Rua Francisco Getúlio Vargas 1130, Petrópolis, 95070-560 Caxias do Sul, RS, Brazil., Garcia PMC; Universidade de Caxias do Sul, Institute of Biotechnology, Rua Francisco Getúlio Vargas 1130, Petrópolis, 95070-560 Caxias do Sul, RS, Brazil.; Brazilian Society of Plastic Surgery - SBCP, Rua Funchal 129, 2º andar, Vila Olímpia, 04551-060 São Paulo, SP, Brazil., Santos OBAF; Brazilian Society of Anesthesiology - SBA, Rua Prof. Alfredo Gomes, 36, Botafogo, 22251-080 Rio de Janeiro, RJ, Brazil., Steffens D; Universidade de Caxias do Sul, Institute of Biotechnology, Rua Francisco Getúlio Vargas 1130, Petrópolis, 95070-560 Caxias do Sul, RS, Brazil., Martins ST; Universidade de Caxias do Sul, Area of Knowledge of Exact Sciences and Engineering, Rua Francisco Getúlio Vargas 1130, Petrópolis, 95070-560 Caxias do Sul, RS, Brazil., Pranke P; Universidade Federal do Rio Grande do Sul, Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Av. Ipiranga, 2752, Azenha, 90610-000 Porto Alegre, RS, Brazil.; Stem Cell Research Institute, R. dos Andradas, 1464 - Conj 133, Centro Histórico, 90050-170 Porto Alegre, RS, Brazil., Crespo JS; Universidade de Caxias do Sul, Area of Knowledge of Exact Sciences and Engineering, Rua Francisco Getúlio Vargas 1130, Petrópolis, 95070-560 Caxias do Sul, RS, Brazil., Henriques JAP; Universidade de Caxias do Sul, Institute of Biotechnology, Rua Francisco Getúlio Vargas 1130, Petrópolis, 95070-560 Caxias do Sul, RS, Brazil., Roesch-Ely M; Universidade de Caxias do Sul, Institute of Biotechnology, Rua Francisco Getúlio Vargas 1130, Petrópolis, 95070-560 Caxias do Sul, RS, Brazil.
Jazyk:	angličtina
Zdroj:	Anais da Academia Brasileira de Ciencias [An Acad Bras Cienc] 2024 Aug 16; Vol. 96 (suppl 1), pp. e20240100. Date of Electronic Publication: 2024 Aug 16 (Print Publication: 2024).
DOI:	10.1590/0001-3765202420240100
Abstrakt:	The use of platelet-rich plasma (PRP) and adipose-derived stromal cells (ADSC) have been investigated as a form of wound healing enhancement. The objective of this work was to evaluate the association of red propolis (RP) and PRP as inducers of ADSC for application in tissue regeneration. Adipose tissue post-collection and post-cryopreservation was isolated with type II collagenase, characterized by flow cytometry, and differentiated into osteogenic, chondrogenic and adipose cell. The viability of ADSC was evaluated when exposed to different concentrations of RP using the MTT and trypan blue assay. Acridine orange and ethidium bromide (AO/EB) was performed to evaluate cell death events. Horizontal migration methods were investigated in ADSC using autologous and homologous PRP associated with RP (PRP/RP). All assays were processed in triplicate. Flow cytometry and cellular differentiation showed that type II collagenase was effective for isolating ADSC post-collection and post-cryopreservation. RP extracts at concentrations of up to 50 μg.mL-1 presented no cytotoxic effects. Association of PRP and RP at 25 and 50 μg.ml-1 influenced ADSC migration, with total closure on the seventh day after exposition. The results here presented could stimulate proliferation of ADSC cells that may contribute directly or indirectly to the reconstructive process of tissue regeneration.
Databáze:	MEDLINE
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