CD4 + T cells exhibit distinct transcriptional phenotypes in the lymph nodes and blood following mRNA vaccination in humans.

Autor: Borcherding N; Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA., Kim W; Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA.; Department of Microbiology, Korea University College of Medicine, Seoul, Korea., Quinn M; Division of Infectious Diseases, Department of Pediatrics, Washington University School of Medicine, Saint Louis, MO, USA., Han F; Department of Emergency Medicine, Washington University School of Medicine, Saint Louis, MO, USA., Zhou JQ; Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA., Sturtz AJ; Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA., Schmitz AJ; Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA., Lei T; Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA., Schattgen SA; Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN, USA., Klebert MK; Clinical Trials Unit, Washington University School of Medicine, Saint Louis, MO, USA., Suessen T; Mallinckrodt Institute of Radiology, Washington University School of Medicine, Saint Louis, MO, USA., Middleton WD; Mallinckrodt Institute of Radiology, Washington University School of Medicine, Saint Louis, MO, USA., Goss CW; Division of Biostatistics, Washington University School of Medicine, Saint Louis, MO, USA., Liu C; Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA., Crawford JC; Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN, USA., Thomas PG; Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN, USA., Teefey SA; Mallinckrodt Institute of Radiology, Washington University School of Medicine, Saint Louis, MO, USA., Presti RM; Clinical Trials Unit, Washington University School of Medicine, Saint Louis, MO, USA.; Division of Infectious Diseases, Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA.; Center for Vaccines and Immunity to Microbial Pathogens, Washington University School of Medicine, Saint Louis, MO, USA.; The Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, Saint Louis, MO, USA., O'Halloran JA; Division of Infectious Diseases, Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA., Turner JS; Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA., Ellebedy AH; Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO, USA. ellebedy@wustl.edu.; Center for Vaccines and Immunity to Microbial Pathogens, Washington University School of Medicine, Saint Louis, MO, USA. ellebedy@wustl.edu.; The Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, Saint Louis, MO, USA. ellebedy@wustl.edu., Mudd PA; Department of Emergency Medicine, Washington University School of Medicine, Saint Louis, MO, USA. pmudd@wustl.edu.; Center for Vaccines and Immunity to Microbial Pathogens, Washington University School of Medicine, Saint Louis, MO, USA. pmudd@wustl.edu.; The Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, Saint Louis, MO, USA. pmudd@wustl.edu.
Jazyk: angličtina
Zdroj: Nature immunology [Nat Immunol] 2024 Sep; Vol. 25 (9), pp. 1731-1741. Date of Electronic Publication: 2024 Aug 20.
DOI: 10.1038/s41590-024-01888-9
Abstrakt: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and mRNA vaccination induce robust CD4 + T cell responses. Using single-cell transcriptomics, here, we evaluated CD4 + T cells specific for the SARS-CoV-2 spike protein in the blood and draining lymph nodes (dLNs) of individuals 3 months and 6 months after vaccination with the BNT162b2 mRNA vaccine. We analyzed 1,277 spike-specific CD4 + T cells, including 238 defined using Trex, a deep learning-based reverse epitope mapping method to predict antigen specificity. Human dLN spike-specific CD4 + follicular helper T (T FH ) cells exhibited heterogeneous phenotypes, including germinal center CD4 + T FH cells and CD4 + IL-10 + T FH cells. Analysis of an independent cohort of SARS-CoV-2-infected individuals 3 months and 6 months after infection found spike-specific CD4 + T cell profiles in blood that were distinct from those detected in blood 3 months and 6 months after BNT162b2 vaccination. Our findings provide an atlas of human spike-specific CD4 + T cell transcriptional phenotypes in the dLNs and blood following SARS-CoV-2 vaccination or infection.
(© 2024. Springer Nature America, Inc.)
Databáze: MEDLINE
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