Molecular characterization and frequency of human pegivirus type 1 (HPgV-1) in kidney transplant recipients from Central-West Brazil.

Autor: da Silva FG; Center for Tropical Medicine, University of Brasília, Federal District, Brasília, Brazil., Moura DR; Center for Tropical Medicine, University of Brasília, Federal District, Brasília, Brazil., de Oliveira PM; Center for Tropical Medicine, University of Brasília, Federal District, Brasília, Brazil.; Molecular Diagnostics Laboratory, University Hospital of Brasília/EBSERH, Federal District, Brasília, Brazil., de Souza Cardoso Quintão T; Center for Tropical Medicine, University of Brasília, Federal District, Brasília, Brazil.; Molecular Diagnostics Laboratory, University Hospital of Brasília/EBSERH, Federal District, Brasília, Brazil., Cassemiro ÉM; Center for Tropical Medicine, University of Brasília, Federal District, Brasília, Brazil.; Molecular Diagnostics Laboratory, University Hospital of Brasília/EBSERH, Federal District, Brasília, Brazil., de Campos GM; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil., da Silva AS; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil., Bezerra RDS; Department of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil., de Noronha LAG; Campus of Ceilândia, University of Brasília, Federal District, Brasília, Brazil., Ramalho WM; Center for Tropical Medicine, University of Brasília, Federal District, Brasília, Brazil.; Campus of Ceilândia, University of Brasília, Federal District, Brasília, Brazil., de Araújo WN; Center for Tropical Medicine, University of Brasília, Federal District, Brasília, Brazil.; Campus of Ceilândia, University of Brasília, Federal District, Brasília, Brazil.; National Institute for Science and Technology for Health Technology Assessment, Porto Alegre, RS, Brazil., Pereira AL; Center for Tropical Medicine, University of Brasília, Federal District, Brasília, Brazil.; Campus of Ceilândia, University of Brasília, Federal District, Brasília, Brazil., Slavov SN; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.; Butantan Institute, São Paulo, Brazil., Haddad R; Center for Tropical Medicine, University of Brasília, Federal District, Brasília, Brazil. haddad@unb.br.; Molecular Diagnostics Laboratory, University Hospital of Brasília/EBSERH, Federal District, Brasília, Brazil. haddad@unb.br.; Campus of Ceilândia, University of Brasília, Federal District, Brasília, Brazil. haddad@unb.br.; Campus Universitário - Centro Metropolitano- DF, Ceilândia Sul, Brasília, DF, CEP: 72220-275, Brazil. haddad@unb.br.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Aug 21. Date of Electronic Publication: 2024 Aug 21.
DOI: 10.1007/s42770-024-01490-z
Abstrakt: Human Pegivirus Type 1 (HPgV-1), a ubiquitous commensal virus, has been recently suggested as a marker of immunologic function. There is scarce data for the presence, genotypes, and molecular characteristics of HPgV-1 among kidney transplant recipients. Therefore, the objective of this study was to examine the prevalence and the molecular characteristics (cycle threshold, genotypes) of this viral infection among kidney transplant recipients from the Brasília, Federal District of Brazil. HPgV-1 RNA detection in the plasma was assessed by RT-qPCR. Positive samples were submitted to sequencing and phylogenetic analysis of the 5´-UTR portion of the viral genome. The estimated HPgV-1 prevalence among renal-transplant recipients was 20%. The performed phylogenetic inference revealed that the most frequent genotype among these patients was HPgV-1 genotype 2 (78.9%) presented by its two subgenotypes (2 A and 2B), followed by genotypes 1 and 3 (10.5% each). This study presents new data about the HPgV-1 circulation and molecular characteristics among kidney transplant recipients from the Federal District of Brazil. Further work is fundamental to examine the effect of HPgV-1 among patients with immunological suppression, including kidney transplant recipients.
(© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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