Efficient and rapid one-step method to generate gene deletions in Streptococcus pyogenes .

Autor:	Schiavolin L; Molecular Bacteriology, European Plotkin Institute for Vaccinology (EPIV), Université libre de Bruxelles, Brussels, Belgium., Lakhloufi D; Molecular Bacteriology, European Plotkin Institute for Vaccinology (EPIV), Université libre de Bruxelles, Brussels, Belgium., Botquin G; Molecular Bacteriology, European Plotkin Institute for Vaccinology (EPIV), Université libre de Bruxelles, Brussels, Belgium., Deneubourg G; Molecular Bacteriology, European Plotkin Institute for Vaccinology (EPIV), Université libre de Bruxelles, Brussels, Belgium., Bruyns C; Molecular Bacteriology, European Plotkin Institute for Vaccinology (EPIV), Université libre de Bruxelles, Brussels, Belgium., Steinmetz J; Molecular Bacteriology, European Plotkin Institute for Vaccinology (EPIV), Université libre de Bruxelles, Brussels, Belgium., Henrot C; Molecular Bacteriology, European Plotkin Institute for Vaccinology (EPIV), Université libre de Bruxelles, Brussels, Belgium., Delforge V; Molecular Bacteriology, European Plotkin Institute for Vaccinology (EPIV), Université libre de Bruxelles, Brussels, Belgium., Smeesters PR; Molecular Bacteriology, European Plotkin Institute for Vaccinology (EPIV), Université libre de Bruxelles, Brussels, Belgium.; Department of Paediatrics, Brussels University Hospital, Academic Children Hospital Queen Fabiola, Université libre de Bruxelles, Brussels, Belgium., Botteaux A; Molecular Bacteriology, European Plotkin Institute for Vaccinology (EPIV), Université libre de Bruxelles, Brussels, Belgium.
Jazyk:	angličtina
Zdroj:	Microbiology spectrum [Microbiol Spectr] 2024 Oct 03; Vol. 12 (10), pp. e0118524. Date of Electronic Publication: 2024 Aug 20.
DOI:	10.1128/spectrum.01185-24
Abstrakt:	Streptococcus pyogenes or Group A Streptococcus (GAS) remains a significant infectious problem around the world, particularly in low- and middle-income settings. Moreover, a recent invasive GAS infection (iGAS) upsurge has been observed in high-income settings. However, to date, no vaccine is available. Finding a good vaccine antigen and understanding the role of virulence factors in GAS infections have been hampered, in part, by technical difficulties to transform the many different strains and generate knockout mutants. Using colE1-type plasmid as a suicide vector, we have set up a method allowing the generation of non-polar mutants of GAS in 3 days. Importance: Group A Streptococcus (GAS) is a major human pathogen, causing diseases ranging from mild and superficial infections of the skin and pharyngeal epithelium to severe systemic and invasive diseases. Since June 2022, several European countries, the US, and Australia are facing an upsurge of invasive life-threatening GAS infections. Finding a good vaccine antigen and understanding the role of virulence factors in GAS infections have been hampered, in part, by technical difficulties to transform the many different GAS strains and generate knockout mutants. Moreover, these tools must be adapted to a large range of different strains, since GAS are divided into more than 260 emm-types (M-type). We have set up a method allowing the generation of non-polar mutants of GAS in 3 days and in diverse backgrounds, which contrasts with previously published protocols. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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