Modeling the roles of cohesotaxis, cell-intercalation, and tissue geometry in collective cell migration of Xenopus mesendoderm.

Autor:	Comlekoglu T; Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA.; Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22903, USA., Dzamba BJ; Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA., Pacheco GG; Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA., Shook DR; Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA., Sego TJ; Department of Medicine, University of Florida, Gainesville, FL 32610, USA., Glazier JA; Department of Intelligent Systems Engineering and The Biocomplexity Institute, Indiana University, Bloomington, IN 47408, USA., Peirce SM; Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22903, USA., DeSimone DW; Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA.
Jazyk:	angličtina
Zdroj:	Biology open [Biol Open] 2024 Aug 15; Vol. 13 (8). Date of Electronic Publication: 2024 Aug 19.
DOI:	10.1242/bio.060615
Abstrakt:	Collectively migrating Xenopus mesendoderm cells are arranged into leader and follower rows with distinct adhesive properties and protrusive behaviors. In vivo, leading row mesendoderm cells extend polarized protrusions and migrate along a fibronectin matrix assembled by blastocoel roof cells. Traction stresses generated at the leading row result in the pulling forward of attached follower row cells. Mesendoderm explants removed from embryos provide an experimentally tractable system for characterizing collective cell movements and behaviors, yet the cellular mechanisms responsible for this mode of migration remain elusive. We introduce a novel agent-based computational model of migrating mesendoderm in the Cellular-Potts computational framework to investigate the respective contributions of multiple parameters specific to the behaviors of leader and follower row cells. Sensitivity analyses identify cohesotaxis, tissue geometry, and cell intercalation as key parameters affecting the migration velocity of collectively migrating cells. The model predicts that cohesotaxis and tissue geometry in combination promote cooperative migration of leader cells resulting in increased migration velocity of the collective. Radial intercalation of cells towards the substrate is an additional mechanism contributing to an increase in migratory speed of the tissue. Model outcomes are validated experimentally using mesendoderm tissue explants. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2024. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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