A micro-scale humanized ventilator-on-a-chip to examine the injurious effects of mechanical ventilation.

Autor:	Gabela-Zuniga B; Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio, USA. ghadiali.1@osu.edu.; The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State Wexner Medical Center, Columbus, Ohio, USA., Shukla VC; Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio, USA. ghadiali.1@osu.edu.; The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State Wexner Medical Center, Columbus, Ohio, USA., Bobba C; Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio, USA. ghadiali.1@osu.edu.; The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State Wexner Medical Center, Columbus, Ohio, USA., Higuita-Castro N; Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio, USA. ghadiali.1@osu.edu.; The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State Wexner Medical Center, Columbus, Ohio, USA.; Department of Neurosurgery, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA., Powell HM; Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio, USA. ghadiali.1@osu.edu.; Department of Materials Science and Engineering, The Ohio State University, Columbus, Ohio, USA.; Scientific Staff, Shriners Children's Ohio, Dayton, Ohio, USA., Englert JA; The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State Wexner Medical Center, Columbus, Ohio, USA.; Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA., Ghadiali SN; Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio, USA. ghadiali.1@osu.edu.; The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State Wexner Medical Center, Columbus, Ohio, USA.; Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.
Jazyk:	angličtina
Zdroj:	Lab on a chip [Lab Chip] 2024 Sep 10; Vol. 24 (18), pp. 4390-4402. Date of Electronic Publication: 2024 Sep 10.
DOI:	10.1039/d4lc00143e
Abstrakt:	Patients with compromised respiratory function frequently require mechanical ventilation to survive. Unfortunately, non-uniform ventilation of injured lungs generates complex mechanical forces that lead to ventilator induced lung injury (VILI). Although investigators have developed lung-on-a-chip systems to simulate normal respiration, modeling the complex mechanics of VILI as well as the subsequent recovery phase is a challenge. Here we present a novel humanized in vitro ventilator-on-a-chip (VOC) model of the lung microenvironment that simulates the different types of injurious forces generated in the lung during mechanical ventilation. We used transepithelial/endothelial electrical impedance measurements to investigate how individual and simultaneous application of mechanical forces alters real-time changes in barrier integrity during and after injury. We find that compressive stress ( i.e. barotrauma) does not significantly alter barrier integrity while over-distention (20% cyclic radial strain, volutrauma) results in decreased barrier integrity that quickly recovers upon removal of mechanical stress. Conversely, surface tension forces generated during airway reopening (atelectrauma), result in a rapid loss of barrier integrity with a delayed recovery relative to volutrauma. Simultaneous application of cyclic stretching (volutrauma) and airway reopening (atelectrauma), indicates that the surface tension forces associated with reopening fluid-occluded lung regions are the primary driver of barrier disruption. Thus, our novel VOC system can monitor the effects of different types of injurious forces on barrier disruption and recovery in real-time and can be used to interogate the biomechanical mechanisms of VILI.
Databáze:	MEDLINE
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