MprF-mediated immune evasion is necessary for Lactiplantibacillus plantarum resilience in the Drosophila gut during inflammation.

Autor: Arias-Rojas A; Research group Genetics of host-microbe interactions, Max Planck Institute for Infection Biology, Berlin, Germany.; Department of Biology, Chemistry, and Pharmacy, Freie Universität Berlin, Berlin, Germany., Arifah AQ; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany., Angelidou G; Core facility for metabolomics and small molecules mass spectrometry, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Alshaar B; Division of Bioanalytical Chemistry, Priority Area Infections, Research Center Borstel, Leibniz Lung Center, Borstel, Germany., Schombel U; Division of Bioanalytical Chemistry, Priority Area Infections, Research Center Borstel, Leibniz Lung Center, Borstel, Germany., Forest E; Research group Genetics of host-microbe interactions, Max Planck Institute for Infection Biology, Berlin, Germany.; CNRS, Aix-Marseille Univ, LISM UMR7255, IMM FR3479, Marseille, France.; Aix Marseille Université, INSERM, SSA, MCT, Marseille, France., Frahm D; Research group Genetics of host-microbe interactions, Max Planck Institute for Infection Biology, Berlin, Germany., Brinkmann V; Microscopy Core Facility, Max Planck Institute for Infection Biology, Berlin, Germany., Paczia N; Core facility for metabolomics and small molecules mass spectrometry, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Beisel CL; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany.; Medical Faculty, University of Würzburg, Würzburg, Germany., Gisch N; Division of Bioanalytical Chemistry, Priority Area Infections, Research Center Borstel, Leibniz Lung Center, Borstel, Germany., Iatsenko I; Research group Genetics of host-microbe interactions, Max Planck Institute for Infection Biology, Berlin, Germany.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2024 Aug 19; Vol. 20 (8), pp. e1012462. Date of Electronic Publication: 2024 Aug 19 (Print Publication: 2024).
DOI: 10.1371/journal.ppat.1012462
Abstrakt: Multiple peptide resistance factor (MprF) confers resistance to cationic antimicrobial peptides (AMPs) in several pathogens, thereby enabling evasion of the host immune response. The role of MprF in commensals remains, however, uncharacterized. To close this knowledge gap, we used a common gut commensal of animals, Lactiplantibacillus plantarum, and its natural host, the fruit fly Drosophila melanogaster, as an experimental model to investigate the role of MprF in commensal-host interactions. The L. plantarum ΔmprF mutant that we generated exhibited deficiency in the synthesis of lysyl-phosphatidylglycerol (Lys-PG), resulting in increased negative cell surface charge and increased susceptibility to AMPs. Susceptibility to AMPs had no effect on ΔmprF mutant's ability to colonize guts of uninfected flies. However, we observed significantly reduced abundance of the ΔmprF mutant after infection-induced inflammation in the guts of wild-type flies but not of flies lacking AMPs. Additionally, we found that the ΔmprF mutant compared to wild-type L. plantarum induces a stronger intestinal immune response in flies due to the increased release of immunostimulatory peptidoglycan fragments, indicating an important role of MprF in promoting host tolerance to commensals. Our further analysis suggests that MprF-mediated lipoteichoic acid modifications are involved in host immunomodulation. Overall, our results demonstrate that MprF, besides its well-characterized role in pathogen immune evasion and virulence, is also an important commensal resilience factor.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Arias-Rojas et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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