Pleomorphic adenoma and carcinoma ex-pleomorphic adenoma tumorigenesis: A proteomic analysis.
| Autor: | Zanella VG; Department of Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.; Head and Neck Surgery Department, Santa Rita Hospital, Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, Brazil., Costa SFDS; Department of Oral Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil., Schuch LF; Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil., Pilar EFS; Experimental Research Unit, Hospital de Clínicas Porto Alegre, Porto Alegre, Brazil., Paes Leme AF; Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil., Dos Santos JN; Postgraduation Program in Dentistry and Health, Federal University of Bahia, Salvador, Brazil., Khurram SA; Academic Unit of Oral and Maxillofacial Medicine and Pathology, Department of Clinical Dentistry, University of Sheffield, Sheffield, UK., Elalawy F; Academic Unit of Oral and Maxillofacial Medicine and Pathology, Department of Clinical Dentistry, University of Sheffield, Sheffield, UK., Bingle L; Academic Unit of Oral and Maxillofacial Medicine and Pathology, Department of Clinical Dentistry, University of Sheffield, Sheffield, UK., Nunes FD; Department of Oral and Maxillofacial Pathology, Dental School, University of São Paulo (USP), São Paulo, Brazil., Fonseca FP; Department of Oral Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil.; Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil., Vargas PA; Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil., Martins MD; Department of Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.; Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil., Wagner VP; Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil.; Academic Unit of Oral and Maxillofacial Medicine and Pathology, Department of Clinical Dentistry, University of Sheffield, Sheffield, UK.; Department of Oral and Maxillofacial Pathology, Dental School, University of São Paulo (USP), São Paulo, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Oral diseases [Oral Dis] 2024 Aug 18. Date of Electronic Publication: 2024 Aug 18. |
| DOI: | 10.1111/odi.15109 |
| Abstrakt: | Objectives: To conduct a comprehensive proteomic analysis of normal salivary gland tissue, pleomorphic adenoma (PA), and carcinoma ex-pleomorphic adenoma (CXPA), and validate the proteomic findings using immunohistochemistry. Methods: Six normal salivary gland tissues, seven PA and seven CXPA samples underwent laser microdissection followed by liquid chromatography coupled to mass spectrometry. Protein identification and quantification were performed using MaxQuant software. Statistical analysis and functional enrichment were conducted using the Perseus platform and STRING tool, respectively. Immunohistochemistry was used for validation. Results: Comparative proteomic analysis revealed 2680 proteins across the three tissue types, with 799 significantly altered between groups. Translocation protein SEC63 homolog, Annexin A6 and Biglycan were up-regulated in CXPA compared to PA. Decorin was markedly up-regulated in both PA and CXPA compared to normal salivary gland (log Conclusions: Proteomic analysis identified differential protein expression patterns associated with malignant transformation of PA into CXPA. Findings indicate a crucial role for extracellular matrix proteins, specifically Biglycan and Decorin, in the tumorigenic progression of PA and CXPA. (© 2024 The Author(s). Oral Diseases published by John Wiley & Sons Ltd.) |
| Databáze: | MEDLINE |
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